The role of P450 metabolism in the estrogenic activity of bifenthrin in fish

Breanna C DeGroot; Susanne M Brander

doi:10.1016/j.aquatox.2014.07.007

The role of P450 metabolism in the estrogenic activity of bifenthrin in fish

Aquat Toxicol. 2014 Nov:156:17-20. doi: 10.1016/j.aquatox.2014.07.007. Epub 2014 Jul 18.

Authors

Breanna C DeGroot¹, Susanne M Brander²

Affiliations

¹ The University of North Carolina at Wilmington, Wilmington, NC, USA. Electronic address: bcd3861@alum.uncw.edu.
² The University of North Carolina at Wilmington, Wilmington, NC, USA. Electronic address: branders@uncw.edu.

PMID: 25127356
DOI: 10.1016/j.aquatox.2014.07.007

Abstract

Bifenthrin, a pyrethroid pesticide, is estrogenic in vivo in fishes. However, bifenthrin is documented to be anti-estrogenic in vitro, in the ER-CALUX (estrogen receptor) cell line. We investigated whether metabolite formation is the reason for this incongruity. We exposed Menidia beryllina (inland silversides) to 10ng/l bifenthrin, 10ng/l 4-hydroxy bifenthrin, and 10ng/l bifenthrin with 25μg/l piperonyl butoxide (PBO) - a P450 inhibitor. Metabolite-exposed juveniles had significantly higher estrogen-mediated protein levels (choriogenin) than bifenthrin/PBO-exposed, while bifenthrin alone was intermediate (not significantly different from either). This suggests that metabolites are the main contributors to bifenthrin's in vivo estrogenicity.

Keywords: 4-hydroxy bifenthrin; ELISA; Endocrine disruption; Environmental estrogen; Menidia beryllina; Pyrethroid.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cytochrome P-450 Enzyme System / metabolism*
Estrogen Antagonists / toxicity
Fish Proteins / metabolism
Fishes / metabolism
Pyrethrins / toxicity*
Water Pollutants, Chemical / toxicity

Substances

Estrogen Antagonists
Fish Proteins
Pyrethrins
Water Pollutants, Chemical
bifenthrin
Cytochrome P-450 Enzyme System