Crystal structure of the homology domain of the eukaryotic DNA replication proteins Sld3/Treslin

Hiroshi Itou; Sachiko Muramatsu; Yasuo Shirakihara; Hiroyuki Araki

doi:10.1016/j.str.2014.07.001

Crystal structure of the homology domain of the eukaryotic DNA replication proteins Sld3/Treslin

Structure. 2014 Sep 2;22(9):1341-1347. doi: 10.1016/j.str.2014.07.001. Epub 2014 Aug 7.

Authors

Hiroshi Itou¹, Sachiko Muramatsu², Yasuo Shirakihara³, Hiroyuki Araki⁴

Affiliations

¹ Structural Biology Center, National Institute of Genetics, Yata1111, Mishima, Shizuoka 411-8540, Japan; Department of Genetics, SOKENDAI, Yata1111, Mishima, Shizuoka 411-8540, Japan. Electronic address: hitou@nig.ac.jp.
² Division of Microbial Genetics, National Institute of Genetics, Yata1111, Mishima, Shizuoka 411-8540, Japan.
³ Structural Biology Center, National Institute of Genetics, Yata1111, Mishima, Shizuoka 411-8540, Japan; Department of Genetics, SOKENDAI, Yata1111, Mishima, Shizuoka 411-8540, Japan.
⁴ Division of Microbial Genetics, National Institute of Genetics, Yata1111, Mishima, Shizuoka 411-8540, Japan; Department of Genetics, SOKENDAI, Yata1111, Mishima, Shizuoka 411-8540, Japan. Electronic address: hiaraki@nig.ac.jp.

PMID: 25126958
DOI: 10.1016/j.str.2014.07.001

Abstract

The initiation of eukaryotic chromosomal DNA replication requires the formation of an active replicative helicase at the replication origins of chromosomal DNA. Yeast Sld3 and its metazoan counterpart Treslin are the hub proteins mediating protein associations critical for the helicase formation. Here, we show the crystal structure of the central domain of Sld3 that is conserved in Sld3/Treslin family of proteins. The domain consists of two segments with 12 helices and is sufficient to bind to Cdc45, the essential helicase component. The structure model of the Sld3-Cdc45 complex, which is crucial for the formation of the active helicase, is proposed.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Cell Cycle Proteins / chemistry*
Cell Cycle Proteins / metabolism
Conserved Sequence
Crystallography, X-Ray
DNA-Binding Proteins / chemistry*
DNA-Binding Proteins / metabolism
Models, Molecular
Molecular Sequence Data
Nuclear Proteins / chemistry
Protein Interaction Domains and Motifs
Protein Structure, Tertiary
Saccharomyces cerevisiae / metabolism
Saccharomyces cerevisiae Proteins / chemistry*
Saccharomyces cerevisiae Proteins / metabolism
Structural Homology, Protein

Substances

CDC45 protein, S cerevisiae
Cell Cycle Proteins
DNA-Binding Proteins
Nuclear Proteins
Saccharomyces cerevisiae Proteins
Sld3 protein, S cerevisiae

Associated data

PDB/3WI3