Molecular cloning and characterization of high mobility group box1 (Ls-HMGB1) from humphead snapper, Lutjanus sanguineus

Jia Cai; Hongli Xia; Yucong Huang; Yishan Lu; Zaohe Wu; Jichang Jian

doi:10.1016/j.fsi.2014.08.004

Molecular cloning and characterization of high mobility group box1 (Ls-HMGB1) from humphead snapper, Lutjanus sanguineus

Fish Shellfish Immunol. 2014 Oct;40(2):539-44. doi: 10.1016/j.fsi.2014.08.004. Epub 2014 Aug 10.

Authors

Jia Cai¹, Hongli Xia¹, Yucong Huang¹, Yishan Lu¹, Zaohe Wu², Jichang Jian³

Affiliations

¹ College of Fishery, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524088, China; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang 524088, China.
² Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524088, China; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang 524088, China; Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
³ College of Fishery, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524088, China; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang 524088, China. Electronic address: jianjc@gmail.com.

PMID: 25120217
DOI: 10.1016/j.fsi.2014.08.004

Abstract

High mobility group box1 (HMGB1) is a kind of chromatin-associated nonhistone protein important for nucleosome formation, transcriptional regulation and inflammation. However, the reports about HMGB1 of marine fish were still limited. Here, we cloned and characterized a HMGB1 gene from humphead snapper, Lutjanus sanguineus (Ls-HMGB1). The Ls-HMGB1 cDNA composed of 1199 bp with a 70 bp of 5'-UTR, 630 bp open reading frame (ORF) and 499 bp 3'-UTR, encoded a polypeptide of 210 amino acids (GenBank Accession No: KJ783442). Sequence alignment of Ls-HMGB1 showed the highest similarity of 91% with Sciaenops ocellatus HMGB1 protein. Quantitative real-time PCR (qRT-PCR) analysis revealed that Ls-HMGB1 had relatively high expression level in skin, kidney and heart. After Vibrio harveyi and poly I:C stimulation, transcripts of Ls-HMGB1 were significantly increased and reached to peak at 18 h p.i. The L. sanguineus interleukin-6 (Ls-IL6) transcription in HK leukocytes was significantly induced by recombinant LsHMGB1 (rLsHMGB1). These results indicated that Ls-HMGB1 may play an important role in immune response of L. sanguineus during pathogen challenge.

Keywords: HMGB1; Humphead snapper; Immune response; Interleukin-6.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adjuvants, Immunologic / pharmacology
Amino Acid Sequence
Animals
Fish Diseases / immunology
Fish Proteins / chemistry
Fish Proteins / genetics*
Fish Proteins / metabolism
Gene Expression Regulation*
HMGB1 Protein / chemistry
HMGB1 Protein / genetics*
HMGB1 Protein / metabolism
Immunity, Innate / drug effects
Interleukin-6 / genetics
Interleukin-6 / metabolism
Molecular Sequence Data
Perciformes / genetics*
Perciformes / immunology
Perciformes / metabolism
Phylogeny
Poly I-C / pharmacology
Real-Time Polymerase Chain Reaction / veterinary
Sequence Alignment
Tissue Distribution
Vibrio / physiology
Vibrio Infections / immunology
Vibrio Infections / veterinary*

Substances

Adjuvants, Immunologic
Fish Proteins
HMGB1 Protein
Interleukin-6
Poly I-C

Associated data

GENBANK/KJ783442