Small-size circulating endothelial microparticles in coronary artery disease

PLoS One. 2014 Aug 12;9(8):e104528. doi: 10.1371/journal.pone.0104528. eCollection 2014.

Abstract

Objectives: Several recent lines of evidence indicate that endothelial microparticles are a new biomarker that can be used to monitor endothelial dysfunction in coronary artery disease (CAD). However, data concerning the detection of small microparticles (diameter <0.5 µm) are lacking. The aim of this study was to detect small-size endothelial microparticles (SEMPs) in CAD patients to monitor endothelial dysfunction.

Methods: In total, 19 CAD patients and 14 healthy subjects were recruited. The absolute numbers and percentages of CD31(+)/CD42b- SEMPs and CD62E(+) SEMPs were determined by flow cytometry. Clinical parameters were also recorded.

Results: The mean percentage of CD62E(+) SEMPs was higher in the CAD patient group than in the healthy subject group. The area under the receiver operating characteristic curve of the percentage of CD62E(+) SEMPs was 0.795, and the cut-off value was 1.35. There was no correlation between the percentage of CD62E(+) SEMPs and various clinical parameters.

Conclusion: The percentage of CD62E(+) SEMPs is a potential biomarker for monitoring endothelial function in CAD.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Biomarkers / blood
  • Biomarkers / metabolism
  • Case-Control Studies
  • Cell-Derived Microparticles / chemistry
  • Cell-Derived Microparticles / metabolism*
  • Coronary Artery Disease / blood
  • Coronary Artery Disease / metabolism*
  • E-Selectin / metabolism
  • Endothelial Cells / metabolism*
  • Endothelium, Vascular / metabolism
  • Female
  • Flow Cytometry
  • Humans
  • Lipids / blood
  • Male
  • Middle Aged
  • ROC Curve
  • Risk Factors

Substances

  • Biomarkers
  • E-Selectin
  • Lipids

Grants and funding

This study is project supported by the National Natural Science Foundation of China (Grant No. 3332013021) & Peking Union Medical College Innovation Funding (No. 3332013021). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.