A simple and sensitive fluorescence based biosensor for the determination of uric acid using H2O2-sensitive quantum dots/dual enzymes

Nur Ellina Azmi; Noor Izaanin Ramli; Jaafar Abdullah; Mohammad Azmi Abdul Hamid; Hamidah Sidek; Samsulida Abd Rahman; Nurhayati Ariffin; Nor Azah Yusof

doi:10.1016/j.bios.2014.07.056

A simple and sensitive fluorescence based biosensor for the determination of uric acid using H2O2-sensitive quantum dots/dual enzymes

Biosens Bioelectron. 2015 May 15:67:129-33. doi: 10.1016/j.bios.2014.07.056. Epub 2014 Jul 30.

Authors

Nur Ellina Azmi¹, Noor Izaanin Ramli², Jaafar Abdullah³, Mohammad Azmi Abdul Hamid⁴, Hamidah Sidek², Samsulida Abd Rahman⁵, Nurhayati Ariffin², Nor Azah Yusof⁶

Affiliations

¹ Industrial Biotechnology Research Centre, SIRIM Berhad, No.1, Persiaran Dato' Menteri, Section 2, P.O. Box 7035, 40700 Shah Alam, Selangor D.E., Malaysia; Institute of Advanced Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor D.E., Malaysia.
² Industrial Biotechnology Research Centre, SIRIM Berhad, No.1, Persiaran Dato' Menteri, Section 2, P.O. Box 7035, 40700 Shah Alam, Selangor D.E., Malaysia.
³ Institute of Advanced Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor D.E., Malaysia; Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor D.E., Malaysia. Electronic address: jafar@upm.edu.my.
⁴ School of Applied Physics, Faculty of Sciences and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor D.E., Malaysia.
⁵ Industrial Biotechnology Research Centre, SIRIM Berhad, No.1, Persiaran Dato' Menteri, Section 2, P.O. Box 7035, 40700 Shah Alam, Selangor D.E., Malaysia; Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor D.E., Malaysia.
⁶ Institute of Advanced Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor D.E., Malaysia; Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor D.E., Malaysia.

PMID: 25113659
DOI: 10.1016/j.bios.2014.07.056

Abstract

A novel optical detection system consisting of combination of uricase/HRP-CdS quantum dots (QDs) for the determination of uric acid in urine sample is described. The QDs was used as an indicator to reveal fluorescence property of the system resulting from enzymatic reaction of uricase and HRP (horseradish peroxidase), which is involved in oxidizing uric acid to allaintoin and hydrogen peroxide. The hydrogen peroxide produced was able to quench the QDs fluorescence, which was proportional to uric acid concentration. The system demonstrated sufficient activity of uricase and HRP at a ratio of 5U:5U and pH 7.0. The linearity of the system toward uric acid was in the concentration range of 125-1000 µM with detection limit of 125 µM.

Keywords: Enzyme; Fluorescence; Quantum dots; Quenching; Uric acid.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biosensing Techniques*
Fluorescence
Fluorescence Resonance Energy Transfer
Horseradish Peroxidase / chemistry
Humans
Hydrogen Peroxide / chemistry
Hydrogen Peroxide / isolation & purification*
Quantum Dots
Urate Oxidase / chemistry
Uric Acid / chemistry
Uric Acid / isolation & purification*
Uric Acid / urine

Substances

Uric Acid
Hydrogen Peroxide
Horseradish Peroxidase
Urate Oxidase