PI3K, MAPK EPK1/2 and p38 are involved in the realization of growth potential of mesenchymal progenitor cells under the influence of basic fibroblast growth factor

G N Zyuz'kov; M G Danilets; A A Ligacheva; V V Zhdanov; E V Udut; L A Miroshnichenko; E V Simanina; A V Chaikovskii; E S Trofimova; M Yu Minakova; V V Udut; A M Dygai

doi:10.1007/s10517-014-2584-z

PI3K, MAPK EPK1/2 and p38 are involved in the realization of growth potential of mesenchymal progenitor cells under the influence of basic fibroblast growth factor

Bull Exp Biol Med. 2014 Aug;157(4):436-9. doi: 10.1007/s10517-014-2584-z. Epub 2014 Aug 12.

Authors

G N Zyuz'kov¹, M G Danilets, A A Ligacheva, V V Zhdanov, E V Udut, L A Miroshnichenko, E V Simanina, A V Chaikovskii, E S Trofimova, M Yu Minakova, V V Udut, A M Dygai

Affiliation

¹ Research Institute of Pharmacology, Siberian Division of the Russian Academy of Medical Sciences, Tomsk, Russia, zgn@pharm.tsu.ru.

PMID: 25113604
DOI: 10.1007/s10517-014-2584-z

Abstract

We studied PI3K, MAPK/EPK1/2 and p38-dependent signaling cascade under stimulation of the functions of mesenchymal progenitor cells by fibroblast growth factor. The important role of PI3K and EPK1/2 in the realization of their growth potential has been revealed. Experiments have demonstrated a blocking effect of PI3K inhibitor on proliferation and differentiation of progenitor cells and EPK1/2 inhibitor on mitotic activity of the progenitor cells. The p38-mediated MAPK pathway did not play a role in determining the proliferative and differentiation status of fibroblast CFU.

MeSH terms

Animals
Bone Marrow Cells / cytology
Bone Marrow Cells / drug effects
Bone Marrow Cells / metabolism
Cell Differentiation / drug effects
Cell Proliferation / drug effects
Fibroblast Growth Factor 2 / pharmacology*
Gene Expression Regulation
Mesenchymal Stem Cells / cytology
Mesenchymal Stem Cells / drug effects*
Mesenchymal Stem Cells / metabolism
Mice
Mice, Inbred CBA
Mitogen-Activated Protein Kinase 1 / antagonists & inhibitors
Mitogen-Activated Protein Kinase 1 / genetics*
Mitogen-Activated Protein Kinase 1 / metabolism
Mitogen-Activated Protein Kinase 3 / antagonists & inhibitors
Mitogen-Activated Protein Kinase 3 / genetics*
Mitogen-Activated Protein Kinase 3 / metabolism
Phosphatidylinositol 3-Kinases / genetics*
Phosphatidylinositol 3-Kinases / metabolism
Phosphoinositide-3 Kinase Inhibitors
Primary Cell Culture
Protein Kinase Inhibitors / pharmacology
Signal Transduction
p38 Mitogen-Activated Protein Kinases / genetics*
p38 Mitogen-Activated Protein Kinases / metabolism

Substances

Phosphoinositide-3 Kinase Inhibitors
Protein Kinase Inhibitors
Fibroblast Growth Factor 2
Mitogen-Activated Protein Kinase 1
Mitogen-Activated Protein Kinase 3
p38 Mitogen-Activated Protein Kinases