Self-assembly of flagellin on Au(111) surfaces

J Colloid Interface Sci. 2014 Nov 1:433:86-93. doi: 10.1016/j.jcis.2014.07.016. Epub 2014 Jul 18.

Abstract

The adsorption of flagellin monomers from Pseudomonas fluorescens on Au(111) has been studied by Atomic Force Microscopy (AFM), Scanning Tunneling Microscopy (STM), X-ray Photoelectron Spectroscopy (XPS), Surface Plasmon Resonance (SPR), and electrochemical techniques. Results show that flagellin monomers spontaneously self-assemble forming a monolayer thick protein film bounded to the Au surface by the more hydrophobic subunit and exposed to the environment the hydrophilic subunit. The films are conductive and allow allocation of electrochemically active cytochrome C. The self-assembled films could be used as biological platforms to build 3D complex molecular structures on planar metal surfaces and to functionalize metal nanoparticles.

Keywords: Cytochrome C; Flagellin; Protein adsorption; Self-assembly.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Flagellin / chemistry*
  • Gold / chemistry*
  • Membranes, Artificial*
  • Metal Nanoparticles / chemistry*
  • Protein Multimerization*
  • Pseudomonas fluorescens / chemistry*
  • Surface Plasmon Resonance / methods

Substances

  • Membranes, Artificial
  • Flagellin
  • Gold