Abstract
The human proto-oncogene product, c-Jun, is a member of the AP-1 family of transcription factors, which mediate the regulation of gene expression in response to extracellular signaling. Comparison of c-Jun and v-Jun by in vitro transcription assays revealed that v-Jun has significantly greater transcriptional activity than c-Jun. Analysis of Jun mutants expressed in bacteria indicates that this difference in transcriptional activity is due to the presence of a regulatory domain located at the N-terminal region of c-Jun. Other Jun mutants identify an activation domain rich in acidic and proline residues toward the C-terminal end of the molecule, in a region near the DNA binding domain. These findings suggest that during retroviral transduction, a constitutively active Jun protein has been generated by deleting a negatively acting domain. This putative repressor domain may also play a role in the signal-dependent induction of c-Jun activity.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Cell Transformation, Viral
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Cloning, Molecular
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Escherichia coli / genetics
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Gene Expression Regulation*
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HeLa Cells / metabolism
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Humans
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Molecular Sequence Data
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Mutation
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Oncogene Protein p65(gag-jun)
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Plasmids
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Protein Processing, Post-Translational
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Protein-Tyrosine Kinases / metabolism
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Proto-Oncogene Mas
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Proto-Oncogene Proteins / metabolism
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Proto-Oncogene Proteins c-jun
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Proto-Oncogenes*
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Retroviridae Proteins, Oncogenic / metabolism*
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Transcription, Genetic*
Substances
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DNA-Binding Proteins
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MAS1 protein, human
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Oncogene Protein p65(gag-jun)
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Proto-Oncogene Mas
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-jun
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Retroviridae Proteins, Oncogenic
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Transcription Factors
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Protein-Tyrosine Kinases