Identification of novel copper/zinc superoxide dismutase (Cu/ZnSOD) genes in kuruma shrimp Marsupenaeus japonicus

Mai Nam Hung; Reina Shiomi; Reiko Nozaki; Hidehiro Kondo; Ikuo Hirono

doi:10.1016/j.fsi.2014.07.030

Identification of novel copper/zinc superoxide dismutase (Cu/ZnSOD) genes in kuruma shrimp Marsupenaeus japonicus

Fish Shellfish Immunol. 2014 Oct;40(2):472-7. doi: 10.1016/j.fsi.2014.07.030. Epub 2014 Aug 8.

Authors

Mai Nam Hung¹, Reina Shiomi¹, Reiko Nozaki¹, Hidehiro Kondo¹, Ikuo Hirono²

Affiliations

¹ Laboratory of Genome Science, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo 108-8477, Japan.
² Laboratory of Genome Science, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo 108-8477, Japan. Electronic address: hirono@kaiyodai.ac.jp.

PMID: 25107696
DOI: 10.1016/j.fsi.2014.07.030

Abstract

Superoxide dismutases (SODs) protect cells from superoxides, but in invertebrates, they also have role in the innate immune system. In this study, the genes for five isoforms of copper/zinc superoxide dismutase (MjCu/ZnSOD) gene were identified and sequenced in kuruma shrimp, Marsupenaeus japonicus. The coding parts of the genes ranged from 516 to 585 bp in length and encoded from 172 to 194 amino acids. Structure, phylogenetic and BLAST analyses indicated that MjCu/ZnSOD isoform_3 and _5 belonged to extracellular Cu/ZnSOD (ecSOD) group while the other three isoforms belong to the intracellular Cu/ZnSOD family. In healthy shrimp, the highest expressions of isoform 2, 3 and 4 were in the gills, whereas the expression of isoform 5 was highest in hemocytes. Challenging the shrimp with WSSV and Vibrio penaeicida up-regulated the mRNA expressions of isoforms 3 and 5, suggesting that these isoforms have roles in the innate immune system of kuruma shrimp.

Keywords: Kuruma shrimp; Vibrio penaeicida; WSSV; cytCu/ZnSOD; ecSOD.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Arthropod Proteins / chemistry
Arthropod Proteins / genetics*
Arthropod Proteins / metabolism
Cloning, Molecular
DNA, Complementary / genetics
DNA, Complementary / metabolism
Molecular Sequence Data
Penaeidae / chemistry
Penaeidae / enzymology*
Penaeidae / genetics*
Penaeidae / immunology
Penaeidae / metabolism
Phylogeny
Protein Isoforms / genetics
Protein Isoforms / metabolism
RNA, Messenger / genetics
RNA, Messenger / metabolism
Sequence Alignment
Superoxide Dismutase / chemistry
Superoxide Dismutase / genetics*
Superoxide Dismutase / metabolism
Tissue Distribution
Vibrio / physiology
White spot syndrome virus 1 / physiology

Substances

Arthropod Proteins
DNA, Complementary
Protein Isoforms
RNA, Messenger
Superoxide Dismutase

Associated data

GENBANK/AB908995
GENBANK/AB908996
GENBANK/AB908997
GENBANK/AB908998
GENBANK/AB908999