Use of multiplex PCR in diagnosis of bloodstream infections in kidney patients

Diagn Microbiol Infect Dis. 2014 Oct;80(2):93-6. doi: 10.1016/j.diagmicrobio.2014.07.001. Epub 2014 Jul 16.

Abstract

The LightCycler® SeptiFast Test (Roche Diagnostics GmbH, Mannheim, Germany) was prospectively compared with the standard blood culture technique in a series of 86 kidney patients. The sensitivity of the PCR compared with the culture was 71%, and the specificity was 88%. All the species identified by culture in these patients were in the SeptiFast panel. The median time to results was 1 day for the PCR, 3 days for positive cultures, and 5 days for negative cultures.

Keywords: Multiplex PCR for diagnostic of sepsis in kidney patients.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Female
  • Humans
  • Kidney Diseases / complications*
  • Male
  • Microbiological Techniques / methods*
  • Middle Aged
  • Molecular Diagnostic Techniques / methods*
  • Multiplex Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Sepsis / diagnosis*
  • Time Factors
  • Young Adult