Study of rust resistance genes in wheat germplasm with DNA markers

Asma Haque; Tayyaba Shaheen; Tahsin Gulzar; Mahmood Ur Rahman; Fatima Jalal; Summera Sattar; Beenish Ehsan; Zafar Iqbal; Muhammad Younas

doi:10.6026/97320630010371

Study of rust resistance genes in wheat germplasm with DNA markers

Bioinformation. 2014 Jun 30;10(6):371-7. doi: 10.6026/97320630010371. eCollection 2014.

Authors

Asma Haque¹, Tayyaba Shaheen¹, Tahsin Gulzar², Mahmood Ur Rahman¹, Fatima Jalal¹, Summera Sattar¹, Beenish Ehsan¹, Zafar Iqbal³, Muhammad Younas³

Affiliations

¹ Department of Bioinformatics and Biotechnology, Government College University, Faisalabad.
² Department of Applied Chemistry, Government College University, Faisalabad.
³ Agriculture Biotechnology Research Institute, AARI, Faisalabad.

Abstract

Wheat is a vital dietary component for human health and widely consumed in the world. Wheat rusts are dangerous pathogens and contribute serious threat to its production. In present study, PCR-Based DNA Markers were employed to check the rust resistance genes among 20 wheat genotypes and 22 markers were amplified. NTSYS-pc 2.2 was used to calculate genetic diversity and Nei and Li's coefficients ranged from 0.55 to 0.95. Cluster analysis was obtained using UPGMA (Unweighted Pair Group Method of Arithmetic Average) algorithm. Maximum no. of genes (23) was amplified from TW-760010 genotype whereas minimum no of genes (14) were amplified from TW-76005 genotype. The data gained from present study open up new ways to produce new varieties by breeding rust resistant germplasm to avoid the economic and food loss and varieties with improved characteristics.

Keywords: Wheat; germplasm; leaf rust; stem rust; stripe rust.