Abstract
To construct a self-assembled biosensor with signal amplification, a cellulosome system, comprising type I and type II dockerin-cohesin interactions with different specificities, from the anaerobic Clostridia bacterium was applied. The self-assembled biosensor was highly sensitive and achieved 128.1-fold increase in detection levels compared to the control.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Bacterial Proteins / chemistry
-
Bacterial Proteins / genetics
-
Bacterial Proteins / metabolism*
-
Biosensing Techniques*
-
Blotting, Western
-
Cell Cycle Proteins / chemistry
-
Cell Cycle Proteins / genetics
-
Cell Cycle Proteins / metabolism*
-
Cellulosomes / chemistry
-
Cellulosomes / metabolism*
-
Chromosomal Proteins, Non-Histone / chemistry
-
Chromosomal Proteins, Non-Histone / genetics
-
Chromosomal Proteins, Non-Histone / metabolism*
-
Clostridium / metabolism*
-
Cohesins
-
Electrophoresis, Polyacrylamide Gel
-
Green Fluorescent Proteins / genetics
-
Green Fluorescent Proteins / metabolism
-
Plasmids / genetics
-
Plasmids / metabolism
-
Polymerase Chain Reaction
-
Protein Binding
Substances
-
Bacterial Proteins
-
Cell Cycle Proteins
-
Chromosomal Proteins, Non-Histone
-
Green Fluorescent Proteins