Signal amplification by a self-assembled biosensor system designed on the principle of dockerin-cohesin interactions in a cellulosome complex

Analyst. 2014 Oct 7;139(19):4790-3. doi: 10.1039/c4an00856a.

Abstract

To construct a self-assembled biosensor with signal amplification, a cellulosome system, comprising type I and type II dockerin-cohesin interactions with different specificities, from the anaerobic Clostridia bacterium was applied. The self-assembled biosensor was highly sensitive and achieved 128.1-fold increase in detection levels compared to the control.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / chemistry
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Biosensing Techniques*
  • Blotting, Western
  • Cell Cycle Proteins / chemistry
  • Cell Cycle Proteins / genetics
  • Cell Cycle Proteins / metabolism*
  • Cellulosomes / chemistry
  • Cellulosomes / metabolism*
  • Chromosomal Proteins, Non-Histone / chemistry
  • Chromosomal Proteins, Non-Histone / genetics
  • Chromosomal Proteins, Non-Histone / metabolism*
  • Clostridium / metabolism*
  • Cohesins
  • Electrophoresis, Polyacrylamide Gel
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • Plasmids / genetics
  • Plasmids / metabolism
  • Polymerase Chain Reaction
  • Protein Binding

Substances

  • Bacterial Proteins
  • Cell Cycle Proteins
  • Chromosomal Proteins, Non-Histone
  • Green Fluorescent Proteins