Neuropeptide degradation by large vessel and microvessel-derived endothelial cells in vitro: cell surface catabolism of thyrotropin releasing hormone (TRH)

Brain Res. 1989 Oct 16;499(2):397-401. doi: 10.1016/0006-8993(89)90792-0.

Abstract

Cell surface ectopeptidase activity of purified, cultured large vessel and microvessel-derived endothelial cells (EC) was studied. Degradation of thyrotropin releasing hormone (TRH), and production of cyclo-His-Pro was significantly increased (P less than 0.001) in large vessel EC compared with microcapillary EC. Since the rate of catabolism in the microvascular capillary bed is 5 times less than that in the large vessel wall, peptide concentrations are likely maintained longer in close proximity to their site of biosynthesis, where they are presumably most active.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Aminopeptidases / metabolism*
  • Animals
  • Cells, Cultured
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / metabolism*
  • Mice
  • Mice, Inbred BALB C
  • Neuropeptides / metabolism*
  • Thyrotropin-Releasing Hormone / metabolism*

Substances

  • Neuropeptides
  • Thyrotropin-Releasing Hormone
  • Aminopeptidases