Abstract
Many applications in biotechnology and molecular biology rely on modified nucleotides. Here, we present an approach for the postsynthetic labelling of enzymatically synthesized vinyl-modified DNA by Diels-Alder reaction with inverse electron demand using a tetrazine. Labelling proceeds very efficiently and supersedes several known approaches.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Biocatalysis
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Cycloaddition Reaction
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DNA / biosynthesis*
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DNA / chemistry
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DNA-Directed DNA Polymerase / metabolism
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Deoxyadenosines / chemistry
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Deoxyadenosines / metabolism
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Deoxyuridine / analogs & derivatives
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Deoxyuridine / chemistry
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Deoxyuridine / metabolism
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Electrons
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Vinyl Compounds / chemistry*
Substances
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Deoxyadenosines
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Vinyl Compounds
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5-vinyl-2'-deoxyuridine
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DNA
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DNA-Directed DNA Polymerase
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Deoxyuridine