Isolation and characterization of bifidobacteria from ovine cheese

Int J Food Microbiol. 2014 Oct 1:188:26-30. doi: 10.1016/j.ijfoodmicro.2014.07.001. Epub 2014 Jul 22.

Abstract

Animal products are one of the niches of bifidobacteria, a fact probably attributable to secondary contamination. In this study, 2 species of the genus Bifidobacterium were isolated by culture-dependent methods from ovine cheeses that were made from unpasteurized milk without addition of starter cultures. The isolates were identified as Bifidobacterium crudilactis and Bifidobacterium animalis subsp. lactis using matrix-assisted laser desorption/ionization time-of-flight analysis and sequencing of phylogenetic markers (16S rRNA, hsp60, and fusA).

Keywords: Bifidobacterium sp.; Cultivation; MALDI-TOF; Ovine cheese; Sequencing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bifidobacterium / classification*
  • Bifidobacterium / genetics
  • Bifidobacterium / isolation & purification*
  • Cheese / microbiology*
  • Food Microbiology*
  • Milk / microbiology
  • Molecular Sequence Data
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Sheep, Domestic

Substances

  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/KJ463393
  • GENBANK/KJ463394
  • GENBANK/KJ463395
  • GENBANK/KJ463396
  • GENBANK/KJ463397
  • GENBANK/KJ463398
  • GENBANK/KJ463399
  • GENBANK/KJ463400
  • GENBANK/KJ463401
  • GENBANK/KJ463402
  • GENBANK/KJ463403
  • GENBANK/KJ463404