Heme oxygenase-1 acts as an antiviral factor for porcine reproductive and respiratory syndrome virus infection and over-expression inhibits virus replication in vitro

Shuqi Xiao; Angke Zhang; Chong Zhang; Huaibo Ni; Jiming Gao; Chengbao Wang; Qin Zhao; Xiangpeng Wang; Xue Wang; Chao Ma; Hongliang Liu; Na Li; Yang Mu; Yani Sun; Gaiping Zhang; Julian A Hiscox; Walter H Hsu; En-Min Zhou

doi:10.1016/j.antiviral.2014.07.011

Heme oxygenase-1 acts as an antiviral factor for porcine reproductive and respiratory syndrome virus infection and over-expression inhibits virus replication in vitro

Antiviral Res. 2014 Oct:110:60-9. doi: 10.1016/j.antiviral.2014.07.011. Epub 2014 Jul 30.

Authors

Shuqi Xiao¹, Angke Zhang¹, Chong Zhang¹, Huaibo Ni¹, Jiming Gao¹, Chengbao Wang¹, Qin Zhao¹, Xiangpeng Wang¹, Xue Wang¹, Chao Ma¹, Hongliang Liu¹, Na Li¹, Yang Mu¹, Yani Sun¹, Gaiping Zhang², Julian A Hiscox³, Walter H Hsu⁴, En-Min Zhou⁵

Affiliations

¹ College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture, China, No. 22 Xinong Road, Yangling, Shaanxi 712100, China.
² College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China; College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan 450002, China.
³ Department of Infection Biology, Institute of Infection and Global Health, University of Liverpool, Liverpool L3 5RF, UK.
⁴ Department of Biomedical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA.
⁵ College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture, China, No. 22 Xinong Road, Yangling, Shaanxi 712100, China. Electronic address: zhouem@nwsuaf.edu.cn.

PMID: 25086213
DOI: 10.1016/j.antiviral.2014.07.011

Abstract

Virus replication depends upon host-cell processes in infected cells, and this is true for porcine reproductive and respiratory syndrome virus (PRRSV), the causative agent of PRRS that is a worldwide threat to the swine industry. Heme oxygenase-1 (HO-1) is a ubiquitously expressed inducible isoform of the first and rate-limiting enzyme for heme degradation. Our previous research suggested that HO-1 may play an important role in PRRSV infection. However, the function of HO-1 in PRRSV infection is unclear. In the present study, Marc-145, PK-15(CD163) cell lines and porcine alveolar macrophages (PAMs) were used to evaluate the effects of HO-1 induction and over-expression on the replication of two different PRRSV strains. Induction of HO-1 markedly decreased the replication of PRRSV strains in the different cells. Similarly, adenoviral-mediated over-expression of HO-1 also greatly decreased the replication of PRRSV. In contrast, ablation of HO-1 using small interfering RNA concomitantly increased PRRSV replication. Therefore, the data were consistent with HO-1 acting as an antiviral factor and these findings suggested that over-expression or induction of HO-1 may provide a potential therapeutic strategy against PRRSV infection.

Keywords: Antivirus factor; HO-1; Highly pathogenic PRRSV; PRRSV; siRNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line
Chlorocebus aethiops
Heme Oxygenase-1 / biosynthesis
Heme Oxygenase-1 / genetics*
Immunity, Innate / genetics
Macrophages, Alveolar / enzymology*
Porcine Reproductive and Respiratory Syndrome / genetics*
Porcine Reproductive and Respiratory Syndrome / therapy
Porcine respiratory and reproductive syndrome virus / growth & development*
RNA Interference
RNA, Small Interfering
Swine
Virus Replication / genetics*

Substances

RNA, Small Interfering
Heme Oxygenase-1