An improved method for the electrotransformation of lactic acid bacteria: A comparative survey

José M Landete; Juan L Arqués; Angela Peirotén; Susana Langa; Margarita Medina

doi:10.1016/j.mimet.2014.07.022

An improved method for the electrotransformation of lactic acid bacteria: A comparative survey

J Microbiol Methods. 2014 Oct:105:130-3. doi: 10.1016/j.mimet.2014.07.022. Epub 2014 Jul 30.

Authors

José M Landete¹, Juan L Arqués², Angela Peirotén², Susana Langa², Margarita Medina²

Affiliations

¹ Departamento de Tecnología de Alimentos, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Carretera de La Coruña km 7.5, 28040 Madrid, Spain. Electronic address: landete.josem@inia.es.
² Departamento de Tecnología de Alimentos, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Carretera de La Coruña km 7.5, 28040 Madrid, Spain.

PMID: 25086179
DOI: 10.1016/j.mimet.2014.07.022

Abstract

An efficient method for genetic transformation of lactic acid bacteria (LAB) by electroporation is presented in this work. A comparative survey with other electrotransformation methods already published showed that the method proposed here yields the higher electrotransformation efficiency in the 12 LAB strains tested, which could make the method applicable to other LAB species or genera.

Keywords: Lactic acid bacteria; Lactobacillus reuteri; Transformation; Voltage.

Publication types

Comparative Study
Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Electroporation / methods*
Genetics, Microbial / methods*
Lactobacillales / genetics*
Molecular Biology / methods*
Transformation, Bacterial*