In vitro efficacy of doxorubicin and etoposide against a feline injection site sarcoma cell line

Joseph Hill; Jessica Lawrence; Corey Saba; Michelle Turek; Brittany Feldhaeusser; Sheryl Coutermarsh-Ott; Jamie Barber; Jo Smith; Robert Gogal Jr

doi:10.1016/j.rvsc.2014.07.006

In vitro efficacy of doxorubicin and etoposide against a feline injection site sarcoma cell line

Res Vet Sci. 2014 Oct;97(2):348-56. doi: 10.1016/j.rvsc.2014.07.006. Epub 2014 Jul 15.

Authors

Joseph Hill¹, Jessica Lawrence², Corey Saba³, Michelle Turek⁴, Brittany Feldhaeusser¹, Sheryl Coutermarsh-Ott⁵, Jamie Barber⁶, Jo Smith³, Robert Gogal Jr⁷

Affiliations

¹ Department of Veterinary Biosciences and Diagnostic Imaging, College of Veterinary Medicine, University of Georgia, Georgia, USA.
² Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Georgia, USA; Veterinary Cancer Centre, The Royal School of Veterinary Studies, University of Edinburgh, UK.
³ Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Georgia, USA.
⁴ Department of Veterinary Biosciences and Diagnostic Imaging, College of Veterinary Medicine, University of Georgia, Georgia, USA; Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Georgia, USA.
⁵ Department of Pathology, College of Veterinary Medicine, University of Georgia, Georgia, USA.
⁶ Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Georgia, USA.
⁷ Department of Veterinary Biosciences and Diagnostic Imaging, College of Veterinary Medicine, University of Georgia, Georgia, USA; Department of Pathology, College of Veterinary Medicine, University of Georgia, Georgia, USA. Electronic address: rgogal@uga.edu.

PMID: 25085537
DOI: 10.1016/j.rvsc.2014.07.006

Abstract

Feline injection site sarcoma (ISS) is a locally invasive tumor, in which surgical treatment is frequently combined with radiation or chemotherapy to improve tumor control. The focus of this study was to evaluate the cytotoxic effects of doxorubicin or etoposide on a feline injection site sarcoma cell line (JB) and to assess the impact of combining these drugs on cell death and cell cycle. Both single agent and combination drug administration increased cell death and significantly reduced the number of viable cells. Cells in G0/G1 were significantly reduced while the G2/M fraction was significantly increased following treatment. Collectively, combining doxorubicin and etoposide at the lower EC yielded comparable results to the EC50 of either drug alone in degree of cytotoxicity, level of apoptosis, and % of cells in G2/M. The results of this study indicate that doxorubicin and etoposide alone and in combination differentially alter ISS cell viability and cycle.

Keywords: Apoptosis; Cell cycle; Doxorubicin; Etoposide; Injection site sarcoma.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antineoplastic Agents / pharmacology
Antineoplastic Agents / therapeutic use
Apoptosis / drug effects*
Cat Diseases / drug therapy
Cat Diseases / pathology*
Cats
Cell Cycle / drug effects
Cell Line, Tumor
Cell Proliferation / drug effects
Cell Survival / drug effects
Doxorubicin / pharmacology*
Doxorubicin / therapeutic use
Drug Therapy, Combination
Etoposide / pharmacology*
Etoposide / therapeutic use
In Vitro Techniques
Neoplasms, Connective Tissue / drug therapy
Neoplasms, Connective Tissue / pathology
Neoplasms, Connective Tissue / veterinary*
Sarcoma / drug therapy
Sarcoma / pathology
Sarcoma / veterinary*

Substances

Antineoplastic Agents
Etoposide
Doxorubicin