Endothelial nitric oxide synthase 894G>T polymorphism and thrombotic disease: a Meta-Analysis of 17 studies involving 8808 subjects

Abstract

Introduction: Endothelial nitric oxide synthase (eNOS) 894G>T polymorphism may influence the risk of thrombotic disease, but data from published studies with low statistical power are inconclusive. To investigate the association between the gene polymorphism and thrombotic disease, a meta-analysis was performed.

Materials and methods: Case-control studies evaluating the association between the eNOS G894T polymorphism, Glu298Asp and thrombotic disease were searched in PubMed, OVID, Web of Science, Google Scholar and China Biology Medicine disc (CBM). Data were available for 4742 cases and 4066 controls from 17 studies.

Results: In all, although there was a significant association between G894T and thrombotic disease (G/T+T/T vs. G/G: OR=1.364, 95%CI=1.126-1.652, P=0.001; T/T vs. G/T+G/G: OR=1.861, 95%CI=1.207-2.870, P=0.005; TT vs. GG: OR=1.938, 95%CI=1.244-3.021, P=0.003; G/T vs. G/G: OR=1.225, 95%CI=1.022-1.469, P=0.028), there was significant heterogeneity among studies (P*<0.001). In subgroup analysis, there was significant association with no heterogeneity in venous thrombosis (G/T+T/T vs. G/G: OR=1.409, 95%CI=1.135-1.750, P=0.002, P*=0.508; T/T vs. G/G: OR=1.640, 95%CI=1.011-2.660, P=0.045, P*=0.333; G/T vs. G/G: OR=1.357, 95%CI=1.082-1.701, P=0.008, P*=0.595) and in Asian population (G/T+T/T vs. G/G: OR=1.722, 95%CI=1.443-2.055, P<0.001, P*=0.541; T/T vs. G/T+G/G: OR=2.357, 95%CI=1.389-4.000, P=0.001, P*=0.908; T/T vs. G/G: OR=2.813, 95%CI=1.645-4.810, P<0.001, P*=0.969; G/T vs. G/G: OR=1.645, 95%CI=1.370-1.975, P<0.001, P*=0.489).

Conclusions: Findings of this meta-analysis demonstrated that eNOS G894T polymorphism may be a risk factor for venous thrombosis, and in Asia the polymorphism may increase the risk of developing thrombotic disease.

Keywords: Atherosclerosis; Meta-analysis; Nitric oxide synthase; Polymorphism; Thrombosis.