[Regulation of Yersinia pseudotuberculosis major porin expression in response to antibiotic stress]

E P Bystritskaia; A M Stenkova; O Iu Portniagina; A V Rakin; V A Rasskazov; M P Isaeva

[Regulation of Yersinia pseudotuberculosis major porin expression in response to antibiotic stress]

Mol Gen Mikrobiol Virusol. 2014:(2):17-21.

[Article in Russian]

Authors

E P Bystritskaia, A M Stenkova, O Iu Portniagina, A V Rakin, V A Rasskazov, M P Isaeva

PMID: 25080814

Abstract

The OmpF porin gene expression in Yersinia pseudotuberculosis in response to antibiotics of two different classes (kanamycin and nalidixic acid) was analyzed using quantitative PCR and a fluorescence reporter system. Both antibiotics downregulated the expression of the ompF gene. The nalidixic acid significantly reduced ompF expression, while kanamycin, for which porins are considered to be an alternative transport route, only slightly reduced the ompF level.

Publication types

English Abstract
Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology*
Bacterial Outer Membrane Proteins / biosynthesis*
Bacterial Outer Membrane Proteins / genetics
Gene Expression Regulation, Bacterial / drug effects*
Gene Expression Regulation, Bacterial / genetics
Kanamycin / pharmacology*
Nalidixic Acid / pharmacology*
Polymerase Chain Reaction
Porins / biosynthesis*
Porins / genetics
Stress, Physiological / drug effects*
Stress, Physiological / genetics
Yersinia pseudotuberculosis / genetics
Yersinia pseudotuberculosis / metabolism*

Substances

Anti-Bacterial Agents
Bacterial Outer Membrane Proteins
OmpF protein
Porins
Nalidixic Acid
Kanamycin