Protein assembly mediated by sulfonatocalix[4]arene

Chem Commun (Camb). 2014 Sep 18;50(72):10412-5. doi: 10.1039/c4cc04897k.

Abstract

A crystal structure of lysozyme in complex with p-sulfonato-calix[4]arene (sclx4) reveals a linear assembly of protein tetramers glued together by protein-calixarene interactions. One interaction involves encapsulation of the highly exposed C-terminal Arg128. The other involves an intricate protein-bound complex of sclx4, Mg(2+) and a fragment of polyethylene glycol.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Calixarenes / chemistry*
  • Calixarenes / metabolism
  • Crystallography, X-Ray
  • Molecular Dynamics Simulation
  • Muramidase / chemistry*
  • Muramidase / metabolism
  • Phenols / chemistry*
  • Phenols / metabolism
  • Polyethylene Glycols / chemistry
  • Polyethylene Glycols / metabolism
  • Protein Binding
  • Protein Structure, Quaternary
  • Sulfonic Acids / chemistry*

Substances

  • Phenols
  • Sulfonic Acids
  • calix(4)arene
  • Calixarenes
  • Polyethylene Glycols
  • Muramidase