Discrepant HIV results resolved by human DNA testing

Julian W Tang; Dena Adachi; Hans Gunning; Carol Marian-Dyer; Stacey Hume; Sherryl Taylor; Shelagh Haase; Leanne Vicen; Marlin Benedet; Anthony Chow; Martin Somerville; Mark Hicks; Graham Tipples

doi:10.1016/j.jcv.2014.06.021

Discrepant HIV results resolved by human DNA testing

J Clin Virol. 2014 Oct;61(2):311-2. doi: 10.1016/j.jcv.2014.06.021. Epub 2014 Jun 30.

Authors

Affiliations

¹ Alberta Provincial Laboratory for Public Health, Edmonton, Alberta, Canada; Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta, Canada. Electronic address: Julian.Tang@albertahealthservices.ca.
² Alberta Provincial Laboratory for Public Health, Edmonton, Alberta, Canada.
³ Department of Medical Genetics, University of Alberta, Edmonton, Alberta, Canada.
⁴ Alberta Provincial Laboratory for Public Health, Edmonton, Alberta, Canada; Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta, Canada.

PMID: 25067806
DOI: 10.1016/j.jcv.2014.06.021

Abstract

A high-risk patient was informed of a positive HIV antibody/antigen test. However, follow-up samples taken 2-3 months later for HIV RNA and anti-HIV antibodies were negative. Human DNA testing confirmed that all samples were from this patient, excluding a sample mix-up. Laboratory investigations revealed a likely splash-over contamination event.

Keywords: Cross-contamination; Forensic; Microsatellite; Mix-up; Sensitivity; Short tandem repeats.

Publication types

Case Reports

MeSH terms

Clinical Laboratory Techniques / methods*
Clinical Laboratory Techniques / standards
Diagnostic Errors*
HIV Antibodies / blood
HIV Antigens / blood
HIV Infections / diagnosis*
Humans
Immunoassay / methods
Male
Molecular Diagnostic Techniques / methods*
RNA, Viral / blood
Young Adult

Substances

HIV Antibodies
HIV Antigens
RNA, Viral