Effects of d-menthol stress on the growth of and microcystin release by the freshwater cyanobacterium Microcystis aeruginosa FACHB-905

Xi Hu; Yunguo Liu; Guangming Zeng; Weihua Xu; Xinjiang Hu; Zhilin Zhu; Pingyang Zhang; Yaqin Wang

doi:10.1016/j.chemosphere.2014.03.084

Effects of d-menthol stress on the growth of and microcystin release by the freshwater cyanobacterium Microcystis aeruginosa FACHB-905

Chemosphere. 2014 Oct:113:30-5. doi: 10.1016/j.chemosphere.2014.03.084. Epub 2014 May 8.

Authors

Xi Hu¹, Yunguo Liu², Guangming Zeng³, Weihua Xu³, Xinjiang Hu¹, Zhilin Zhu⁴, Pingyang Zhang¹, Yaqin Wang¹

Affiliations

¹ College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China.
² College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control, Hunan University, Ministry of Education, Changsha 410082, PR China. Electronic address: xixixixi2046@gmail.com.
³ College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control, Hunan University, Ministry of Education, Changsha 410082, PR China.
⁴ Key Laboratory of Environmental Biology and Pollution Control, Hunan University, Ministry of Education, Changsha 410082, PR China.

PMID: 25065786
DOI: 10.1016/j.chemosphere.2014.03.084

Abstract

The effects of d-menthol on the growth of Microcystis aeruginosa FACHB-905 and microcystin (MCY) concentration were evaluated by batch culture experiments. The algal biomass and the intracellular and extracellular MCY concentrations were evaluated during 5d incubation. After the d-menthol exposure, the dry weight of the cells gradually decreased; the decrease in the dry weight after 5d exposure was 29, 12, and 2mgL(-1) when the initial cell densities were 1.4×10(7), 1.2×10(6), and 2.9×10(5)cellmL(-1), respectively. The results indicate that the d-menthol exposure inhibited the cellular growth, thus also inhibiting the increase of the total MCY concentration. In the presence of d-menthol, the intracellular MCY was gradually released into the medium after the cell lysis. The extracellular MCY concentration in the medium was significantly higher in the d-menthol-exposed samples than in the control samples, confirming that d-menthol cannot decompose the extracellular MCY.

Keywords: Microcystin; Microcystis aeruginosa; Protein pattern; d-Menthol.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Analysis of Variance
Electrophoresis, Polyacrylamide Gel
Fresh Water / microbiology
Menthol / toxicity*
Microcystins / metabolism*
Microcystis / drug effects*
Microcystis / growth & development
Microcystis / metabolism

Substances

Microcystins
Menthol
microcystin