Nano-scale liquid chromatography coupled to tandem mass spectrometry using the multiple reaction monitoring mode based quantitative platform for analyzing multiple enzymes associated with central metabolic pathways of Saccharomyces cerevisiae using ultra fast mass spectrometry

Fumio Matsuda; Tairo Ogura; Atsumi Tomita; Ichiro Hirano; Hiroshi Shimizu

doi:10.1016/j.jbiosc.2014.06.010

Nano-scale liquid chromatography coupled to tandem mass spectrometry using the multiple reaction monitoring mode based quantitative platform for analyzing multiple enzymes associated with central metabolic pathways of Saccharomyces cerevisiae using ultra fast mass spectrometry

J Biosci Bioeng. 2015 Jan;119(1):117-20. doi: 10.1016/j.jbiosc.2014.06.010. Epub 2014 Jul 21.

Authors

Fumio Matsuda¹, Tairo Ogura², Atsumi Tomita³, Ichiro Hirano⁴, Hiroshi Shimizu⁵

Affiliations

¹ Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka, Japan; RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan. Electronic address: fmatsuda@ist.osaka-u.ac.jp.
² Mass Spectrometry Business Unit, Shimadzu Corporation, 1, Nishinokyo Kuwabara-cho, Nakagyo-ku, Kyoto 604-8511, Japan. Electronic address: ogutairo@shimadzu.co.jp.
³ Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka, Japan. Electronic address: atsumi_tomita@bio.eng.osaka-u.ac.jp.
⁴ Mass Spectrometry Business Unit, Shimadzu Corporation, 1, Nishinokyo Kuwabara-cho, Nakagyo-ku, Kyoto 604-8511, Japan. Electronic address: i_hirano@shimadzu.co.jp.
⁵ Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka, Japan. Electronic address: shimizu@bio.eng.osaka-u.ac.jp.

PMID: 25060728
DOI: 10.1016/j.jbiosc.2014.06.010

Abstract

A widely targeted quantitative proteome analysis of Saccharomyces cerevisiae enzymes was performed employing an ultra fast mass spectrometry (UFMS). Nano-liquid chromatography-UFMS analysis of trypsin digested peptide samples derived from yeast strains successfully determined relative abundances of 303 peptides of 137 proteins.

Keywords: Central carbon metabolism; Enzyme expression; Quantitative proteomics; Saccharomyces cerevisiae; Ultra fast mass spectrometry.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, Liquid
Mass Spectrometry / methods*
Metabolic Networks and Pathways*
Peptides / metabolism
Proteome / metabolism
Proteomics
Saccharomyces cerevisiae / enzymology
Saccharomyces cerevisiae / metabolism*
Saccharomyces cerevisiae Proteins / chemistry
Saccharomyces cerevisiae Proteins / metabolism
Tandem Mass Spectrometry
Trypsin / metabolism

Substances

Peptides
Proteome
Saccharomyces cerevisiae Proteins
Trypsin