Affinity chemiresistor sensor for sugars

Chaker Tlili; Sushmee Badhulika; Thien-Toan Tran; Ilkeun Lee; Ashok Mulchandani

doi:10.1016/j.talanta.2014.05.055

Affinity chemiresistor sensor for sugars

Talanta. 2014 Oct:128:473-9. doi: 10.1016/j.talanta.2014.05.055. Epub 2014 Jun 6.

Authors

Chaker Tlili¹, Sushmee Badhulika², Thien-Toan Tran³, Ilkeun Lee⁴, Ashok Mulchandani⁵

Affiliations

¹ Department of Chemical and Environmental Engineering, University of California, Riverside, CA 92521, USA. Electronic address: chaker_tlili@yahoo.fr.
² Department of Electrical Engineering, University of California, Riverside, CA 92521, USA. Electronic address: sbadh001@ucr.edu.
³ Department of Bioengineering, University of California, Riverside, CA 92521, USA. Electronic address: ttran0096@ucr.edu.
⁴ Department of Chemistry, University of California, Riverside, CA 92521, USA. Electronic address: ilkeun.lee@ucr.edu.
⁵ Department of Chemical and Environmental Engineering, University of California, Riverside, CA 92521, USA. Electronic address: adani@engr.ucr.edu.

PMID: 25059188
DOI: 10.1016/j.talanta.2014.05.055

Abstract

In this work, a non-enzymatic chemiresistive sugar sensor has been developed by combining a synthetic receptor with aligned single-walled carbon nanotubes (SWNTs) device. Briefly, boronic acid as a multivalent sugar receptor was immobilized on carbon nanotubes through amide bond formation. The interaction between three common sugars (d-glucose, d-fructose and sucrose) and boronic acid modified SWNTs device was studied. The effect of pH on the receptor-ligand binding was examined and highest response was observed at pH 9. The chemiresistive sensor exhibited specific and reproducible detection with sensitivity over the concentration range of 1-20mM, 1-25 mM, and 1-30 mM for fructose, glucose, and sucrose, respectively. The sensor showed no interference from common electroactive compounds such as citric acid, uric acid, and ascorbic acid. Furthermore, the sensor retained 97.4% of the initial value after five regeneration cycles with an acidic buffer at pH 5, thus ensuring good reusability.

Keywords: Boronic acid; Non-enzymatic sensor; Single-walled carbon nanotubes; Sugar.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Biosensing Techniques / methods*
Boronic Acids / chemistry*
Carbohydrates / analysis*
Carbohydrates / chemistry
Fructose / analysis
Fructose / chemistry
Glucose / analysis
Glucose / chemistry
Hydrogen-Ion Concentration
Nanotubes, Carbon / chemistry*
Reproducibility of Results
Sucrose / analysis
Sucrose / chemistry

Substances

Boronic Acids
Carbohydrates
Nanotubes, Carbon
Fructose
Sucrose
Glucose