Up-regulation of 91H promotes tumor metastasis and predicts poor prognosis for patients with colorectal cancer

Qiwen Deng; Bangshun He; Tianyi Gao; Yuqin Pan; Huiling Sun; Yeqiong Xu; Rui Li; Houqun Ying; Feng Wang; Xian Liu; Jie Chen; Shukui Wang

doi:10.1371/journal.pone.0103022

Up-regulation of 91H promotes tumor metastasis and predicts poor prognosis for patients with colorectal cancer

PLoS One. 2014 Jul 24;9(7):e103022. doi: 10.1371/journal.pone.0103022. eCollection 2014.

Authors

Qiwen Deng¹, Bangshun He¹, Tianyi Gao¹, Yuqin Pan¹, Huiling Sun², Yeqiong Xu¹, Rui Li², Houqun Ying³, Feng Wang¹, Xian Liu¹, Jie Chen², Shukui Wang¹

Affiliations

¹ Central Laboratory, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.
² Department of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu, China.
³ Medical college, Southeast University, Nanjing, Jiangsu, China.

Abstract

Background: Long noncoding RNAs (lncRNAs) play widespread roles in gene regulation and cellular processes. However, the functional roles of lncRNAs in colorectal cancer (CRC) are not yet well elucidated. The aim of the present study was to measure the levels of lncRNA 91H expression in CRC and evaluate its clinical significance and biological roles in the development and progression of CRC.

Methods: 91H expression and copy number variation (CNV) were measured in 72 CRC tumor tissues and adjacent normal tissues by real-time PCR. The biological roles of 91H were evaluated by MTT, scratch wound assay, migration and invasion assays, and flow cytometry.

Results: 91H was significantly overexpressed in cancerous tissue and CRC cell lines compared with adjacent normal tissue and a normal human intestinal epithelial cell line. Moreover, 91H overexpression was closely associated with distant metastasis and poor prognosis in patients with CRC, except for CNV of 91H. Multivariate analysis indicated that 91H expression was an independent prognostic indicator, as well as distant metastasis. Our in vitro data indicated that knockdown of 91H inhibited the proliferation, migration, and invasiveness of CRC cells.

Conclusions: 91H played an important role in the molecular etiology of CRC and might be regarded as a novel prognosis indicator in patients with CRC.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aged
Aged, 80 and over
Biomarkers, Tumor / genetics
Cell Line
Colorectal Neoplasms / diagnosis*
Colorectal Neoplasms / genetics
Colorectal Neoplasms / mortality*
DNA Copy Number Variations
Female
Gene Expression Regulation, Neoplastic
HCT116 Cells
HT29 Cells
Humans
Male
Middle Aged
Neoplasm Metastasis
Prognosis
RNA, Long Noncoding / genetics*
Survival Analysis
Up-Regulation / genetics

Substances

Biomarkers, Tumor
RNA, Long Noncoding

Grants and funding

This project was supported by grants from The National Nature Science Foundation of China (no. 81172141, 81200401), Nanjing Science and Technology Committee Project (no.201108025), Nanjing Medical Technology Development Project (no. ZKX11025), Nanjing Health Young Talent Project, Jiangsu Provincial Key Medical Talents to S.K.W., Nanjing Medical Science and Technique Development Foundation to Y.Q.P. (no. QRX11255) and B.S.H. (no. QRX11254). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.