Abstract
Previously developed radical-scavenging pea protein hydrolysates (PPHs) prepared with Flavourzyme (Fla-PPH) and Protamex (Pro-PPH) were used as cosurfactants with Tween 20 to produce soybean oil-in-water (O/W) emulsions, and the suppression of lipid oxidation was investigated. Both PPHs significantly retarded oxidation (P < 0.05) of the emulsions when stored at 37 °C for 14 days. Electron microscopy revealed an interfacial peptidyl membrane around oil droplets, which afforded steric restrictions to oxidation initiators. When licorice extract (LE) was also used in emulsion preparation, a remarkable synergistic oxidation inhibition was observed with both PPHs. LE adsorbed onto oil droplets either directly or through associating with PPH to produce a thick and compact interfacial membrane enabling the defense against oxygen species. Liquiritin apioside, neolicuroside, glabrene, and 18β-glycyrrhetic acid were the predominant phenolic derivatives partitioning at the interface and most likely the major contributors to the notable synergistic antioxidant activity when coupled with PPHs.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Antioxidants / chemistry*
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Emulsifying Agents / agonists
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Emulsifying Agents / chemistry
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Emulsifying Agents / metabolism
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Emulsions
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Endopeptidases / metabolism
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Flavonoids / agonists
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Flavonoids / chemistry
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Food Preservatives / chemistry
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Food Preservatives / metabolism
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Food Storage
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Glucosides / agonists
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Glucosides / chemistry
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Glycyrrhiza / chemistry*
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Oxidation-Reduction
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Pisum sativum / chemistry*
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Plant Extracts / agonists
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Plant Extracts / chemistry*
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Plant Proteins, Dietary / agonists
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Plant Proteins, Dietary / chemistry*
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Plant Proteins, Dietary / metabolism
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Plant Roots / chemistry
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Protein Hydrolysates / agonists
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Protein Hydrolysates / chemistry*
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Protein Stability
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Seed Storage Proteins / agonists
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Seed Storage Proteins / chemistry
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Seed Storage Proteins / metabolism
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Seeds / chemistry
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Soybean Oil / chemistry*
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Surface Properties
Substances
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Antioxidants
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Emulsifying Agents
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Emulsions
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Flavonoids
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Food Preservatives
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Glucosides
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Plant Extracts
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Plant Proteins, Dietary
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Protein Hydrolysates
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Seed Storage Proteins
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Soybean Oil
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Endopeptidases
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flavourzyme