Precursor ion scan driven fast untargeted screening and semi-determination of caffeoylquinic acid derivatives in Cynara scolymus L

Qing Shen; Yanbin Lu; Zhiyuan Dai; Hon-Yeung Cheung

doi:10.1016/j.foodchem.2014.06.030

Precursor ion scan driven fast untargeted screening and semi-determination of caffeoylquinic acid derivatives in Cynara scolymus L

Food Chem. 2015 Jan 1:166:442-447. doi: 10.1016/j.foodchem.2014.06.030. Epub 2014 Jun 14.

Authors

Qing Shen¹, Yanbin Lu², Zhiyuan Dai³, Hon-Yeung Cheung⁴

Affiliations

¹ College of Food Science and Technology, Zhejiang Gongshang University, Hangzhou, China; Department of Biomedical Sciences, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong Special Administrative Region.
² College of Food Science and Technology, Zhejiang Gongshang University, Hangzhou, China.
³ College of Food Science and Technology, Zhejiang Gongshang University, Hangzhou, China. Electronic address: dzy@zjgsu.edu.cn.
⁴ Department of Biomedical Sciences, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong Special Administrative Region. Electronic address: bhhonyun@cityu.edu.hk.

PMID: 25053078
DOI: 10.1016/j.foodchem.2014.06.030

Abstract

A precursor ion scan (PIS) technique based strategy was developed for rapid screening and semi-determination of caffeoylquinic acid derivatives (CADs) in artichoke (Cynara scolymus L.) using ultra-performance liquid chromatography (UPLC) coupled with tandem mass spectrometry. 1,5-Dicaffeoylquinic acid and 5-caffeoylquinic acid were used for studying the fragmentation behaviour of two classes of CADs, setting m/z 191 as a diagnostic moiety. When it was applied to artichoke sample, ten CADs were detected and elucidated in a single PIS run. Furthermore, method validation was implemented including: specificity (no interference), linearity (≥0.9993), limit of detection (LOD<0.12 ng mL(-1)) and limit of quantification (LOQ<0.25 ng mL(-1)), precision (RSD≤3.6), recovery (91.4-95.9%) and stability (at least 12 h). This approach was proven to be a powerful, selective and sensitive tool for rapid screening and semi-determination of untargeted components in natural products.

Keywords: Caffeoylquinic acid derivative; Cynara scolymus L.; Precursor ion scan; UPLC–MS/MS.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, Liquid / methods
Cynara scolymus / chemistry*
Quinic Acid / analogs & derivatives*
Quinic Acid / chemistry
Tandem Mass Spectrometry / methods*

Substances

caffeoylquinic acid
Quinic Acid