Abstract
cAMPhor: In the presence of cAMP, cAMPhor folds into a structure that binds DFHBI (green), increasing its fluorescence, while Alexa 647 (red) functions as a normalizing dye. It can thus be used to spatially image cAMP quantitatively in membrane-bound compartments.
Keywords:
DFHBI; GUVs; aptamers; cAMP; fluorescence; sensors.
© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Benzyl Compounds / chemistry
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Biosensing Techniques / instrumentation*
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Biosensing Techniques / methods
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Cell Membrane / metabolism*
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Cell Membrane / microbiology
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Cell Membrane / pathology
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Cyclic AMP / analysis*
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Cyclic AMP / metabolism*
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Dimyristoylphosphatidylcholine / chemistry
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Fluorescence
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Host-Pathogen Interactions
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Imidazolines / chemistry
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Lipid Bilayers / chemistry
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Lipid Bilayers / metabolism
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Microscopy, Fluorescence / instrumentation
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Microscopy, Fluorescence / methods
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Mycobacterium Infections, Nontuberculous / metabolism
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Mycobacterium Infections, Nontuberculous / pathology
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Mycobacterium smegmatis / physiology
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Nanostructures / chemistry*
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Nucleic Acid Conformation
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Nucleic Acids / chemistry*
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Optical Imaging / instrumentation*
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Optical Imaging / methods
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RNA / chemistry
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Sensitivity and Specificity
Substances
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3,5-difluoro-4-hydroxybenzylidene imidazolinone
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Benzyl Compounds
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Imidazolines
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Lipid Bilayers
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Nucleic Acids
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RNA
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Cyclic AMP
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Dimyristoylphosphatidylcholine