A fluorescent nucleic acid nanodevice quantitatively images elevated cyclic adenosine monophosphate in membrane-bound compartments

Small. 2014 Nov 12;10(21):4276-80. doi: 10.1002/smll.201400833. Epub 2014 Jul 14.

Abstract

cAMPhor: In the presence of cAMP, cAMPhor folds into a structure that binds DFHBI (green), increasing its fluorescence, while Alexa 647 (red) functions as a normalizing dye. It can thus be used to spatially image cAMP quantitatively in membrane-bound compartments.

Keywords: DFHBI; GUVs; aptamers; cAMP; fluorescence; sensors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Benzyl Compounds / chemistry
  • Biosensing Techniques / instrumentation*
  • Biosensing Techniques / methods
  • Cell Membrane / metabolism*
  • Cell Membrane / microbiology
  • Cell Membrane / pathology
  • Cyclic AMP / analysis*
  • Cyclic AMP / metabolism*
  • Dimyristoylphosphatidylcholine / chemistry
  • Fluorescence
  • Host-Pathogen Interactions
  • Imidazolines / chemistry
  • Lipid Bilayers / chemistry
  • Lipid Bilayers / metabolism
  • Microscopy, Fluorescence / instrumentation
  • Microscopy, Fluorescence / methods
  • Mycobacterium Infections, Nontuberculous / metabolism
  • Mycobacterium Infections, Nontuberculous / pathology
  • Mycobacterium smegmatis / physiology
  • Nanostructures / chemistry*
  • Nucleic Acid Conformation
  • Nucleic Acids / chemistry*
  • Optical Imaging / instrumentation*
  • Optical Imaging / methods
  • RNA / chemistry
  • Sensitivity and Specificity

Substances

  • 3,5-difluoro-4-hydroxybenzylidene imidazolinone
  • Benzyl Compounds
  • Imidazolines
  • Lipid Bilayers
  • Nucleic Acids
  • RNA
  • Cyclic AMP
  • Dimyristoylphosphatidylcholine