ENU Mutagenesis in the Mouse

Abstract

This unit describes the treatment of laboratory mice with the mutagen N-ethyl-N-nitrosourea (ENU) to induce very highly increased rates of mutation throughout the genome. Further, it describes several popular mating schemes designed to produce animals displaying phenotypes associated with the induced mutations.

Keywords: alkylating agent; genome sequencing; per locus mutation frequency; phenotype-driven mutagenesis; spermatogonial stem cells.