A rapid and sensitive LC-MS/MS method for the determination of Pulsatilla saponin D in rat plasma and its application in a rat pharmacokinetic and bioavailability study

Biomed Chromatogr. 2015 Mar;29(3):373-8. doi: 10.1002/bmc.3286. Epub 2014 Jul 9.

Abstract

A simple, sensitive and specific liquid chromatography-tandem mass spectrometry method was developed and validated for the determination of Pulsatilla saponin D, a potential antitumor constituent isolated from Pulsatilla chinensis in rat plasma. Rat plasma samples were pretreated by protein precipitation with methanol. The method validation was performed in accordance with US Food and Drug Administration guidelines and the results met the acceptance criteria. The method was successfully applied to assess the pharmacokinetics and oral bioavailability of Pulsatilla saponin D in rats.

Keywords: LC-MS/MS; Pulsatilla saponin D; bioavailability; pharmacokinetics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Oral
  • Animals
  • Antineoplastic Agents, Phytogenic / analysis
  • Antineoplastic Agents, Phytogenic / pharmacokinetics
  • Biological Availability
  • Chemical Precipitation
  • Chromatography, High Pressure Liquid / methods
  • Drug Stability
  • Male
  • Oleanolic Acid / analogs & derivatives
  • Oleanolic Acid / analysis
  • Oleanolic Acid / pharmacokinetics
  • Pulsatilla / chemistry*
  • Rats, Sprague-Dawley
  • Reproducibility of Results
  • Saponins / administration & dosage
  • Saponins / analysis
  • Saponins / blood*
  • Saponins / pharmacokinetics*
  • Sensitivity and Specificity
  • Tandem Mass Spectrometry / methods*
  • United States
  • United States Food and Drug Administration

Substances

  • Antineoplastic Agents, Phytogenic
  • Pulsatilla saponin D
  • Saponins
  • saponin D, hupehensis
  • Oleanolic Acid