Phenolic profile, antioxidant, anti-inflammatory and cytotoxic activities of black (Tuber aestivum Vittad.) and white (Tuber magnatum Pico) truffles

Ivana N Beara; Marija M Lesjak; Dragana D Cetojević-Simin; Zaklina S Marjanović; Jelena D Ristić; Zorica O Mrkonjić; Neda M Mimica-Dukić

doi:10.1016/j.foodchem.2014.05.116

Phenolic profile, antioxidant, anti-inflammatory and cytotoxic activities of black (Tuber aestivum Vittad.) and white (Tuber magnatum Pico) truffles

Food Chem. 2014 Dec 15:165:460-6. doi: 10.1016/j.foodchem.2014.05.116. Epub 2014 Jun 2.

Authors

Ivana N Beara¹, Marija M Lesjak², Dragana D Cetojević-Simin³, Zaklina S Marjanović⁴, Jelena D Ristić², Zorica O Mrkonjić², Neda M Mimica-Dukić²

Affiliations

¹ Department of Chemistry, Biochemistry and Environmental Protection, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovića 3, 21000 Novi Sad, Serbia. Electronic address: ivana.beara@dh.uns.ac.rs.
² Department of Chemistry, Biochemistry and Environmental Protection, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovića 3, 21000 Novi Sad, Serbia.
³ Oncology Institute of Vojvodina, Faculty of Medicine, University of Novi Sad, Dr Goldmana 4, 21204 Sremska Kamenica, Serbia.
⁴ Institute for Multidisciplinary Research, University of Belgrade, Kneza Višeslava 1a, 11 000 Beograd, Serbia.

PMID: 25038699
DOI: 10.1016/j.foodchem.2014.05.116

Abstract

The aim of this study was a comprehensive investigation on phenolic profile, antioxidant, anti-inflammatory and cytotoxic activity of the still insufficiently explored black summer truffles (Tuber aestivum Vittad.) and white (Tuber magnatum Pico) truffles. Methanol and water extracts, obtained by maceration and Soxhlet extraction, were examined. Forty-five phenolics was studied using LC-MS/MS: presence of 14 compounds was confirmed, with the most dominant being p-hydroxybenzoic acid, baicalein and kaempferol (T. aestivum), epicatechin and catechin (T. magnatum). Moderate antioxidant activity of both species was determined through several assays. Only T. magnatum showed anti-inflammatory potential by inhibiting COX-1 and 12-LOX pathway products synthesis. Methanol extracts exerted cytotoxicity against some tumour cell lines (HeLa, MCF7, HT-29), besides the prominent activity of water extracts towards breast adenocarcinoma (MCF7). To conclude, these results support further investigations of phytochemicals and biological activity towards verification of nutraceutical use of both species.

Keywords: Anti-inflammatory activity; Antioxidant activity; Cytotoxic activity; LC–MS/MS; Tuber aestivum; Tuber magnatum.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Inflammatory Agents / chemistry*
Antioxidants / chemistry*
Ascomycota / chemistry*
Phenols / chemistry*
Tandem Mass Spectrometry

Substances

Anti-Inflammatory Agents
Antioxidants
Phenols