Transcriptional regulation of gene expression of infectious salmon anaemia virus segment 7

Virus Res. 2014 Sep 22:190:69-74. doi: 10.1016/j.virusres.2014.07.008. Epub 2014 Jul 16.

Abstract

The nuclear replication and gene splicing of orthomyxoviruses are unique among RNA viruses. Segment 7 of infectious salmon anaemia virus (ISAV) is the only segment that undergoes splicing. Two proteins are encoded by this segment, the non-structural antagonist (ISAV-NS) of the innate immune response that is translated from the unspliced collinear transcript, and a nuclear exporting protein (ISAV-NEP) that is translated from the spliced mRNA. Here we report the transcription profiles for these ISAV proteins. The appearance of the spliced ISAV-NEP mRNA was delayed and the relative amount was less but slowly accumulated to 20-30% to that of the collinear NS mRNA. In cells transfected with segment 7 the ratio between spliced and collinear mRNA was approximately 10%. A highly conserved, possible structured RNA, in the region of the 3' splicing site of the segment is speculated as being important for the regulation of the efficiency of the splicing.

Keywords: Gene expression of NS and NEP; Infectious salmon anaemia virus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Fish Diseases / virology*
  • Gene Expression Regulation, Viral*
  • Isavirus / genetics*
  • Isavirus / metabolism
  • Molecular Sequence Data
  • Orthomyxoviridae Infections / veterinary*
  • Orthomyxoviridae Infections / virology
  • RNA Splicing
  • RNA, Viral / genetics
  • Salmon / virology
  • Transcription, Genetic*
  • Viral Proteins / genetics
  • Viral Proteins / metabolism

Substances

  • RNA, Viral
  • Viral Proteins