Up-regulation of nuclear factor E2-related factor 2 upon SVCV infection

Yi Yang; Jian Huang; Lijuan Li; Li Lin; Yanhua Zhai; Xiaoxuan Chen; Xueqin Liu; Zhixin Wu; Junfa Yuan

doi:10.1016/j.fsi.2014.07.015

Up-regulation of nuclear factor E2-related factor 2 upon SVCV infection

Fish Shellfish Immunol. 2014 Sep;40(1):245-52. doi: 10.1016/j.fsi.2014.07.015. Epub 2014 Jul 17.

Authors

Yi Yang¹, Jian Huang², Lijuan Li², Li Lin², Yanhua Zhai³, Xiaoxuan Chen², Xueqin Liu¹, Zhixin Wu⁴, Junfa Yuan⁵

Affiliations

¹ Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China; Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, 430070, China.
² Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, 430070, China.
³ Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China.
⁴ Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, 430070, China. Electronic address: wuzhixin@mail.hzau.edu.cn.
⁵ Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, 430070, China; Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, 430070, China. Electronic address: jfyuan@mail.hzau.edu.cn.

PMID: 25038284
DOI: 10.1016/j.fsi.2014.07.015

Abstract

Nuclear factor E2 - related factor 2 (Nrf2) is a crucial transcription factor that regulates the basal and inducible expression of many antioxidant response element (ARE)-dependent genes, including heme oxygenase-1 (HO-1) and superoxide dismutase 1 (SOD1). The Nrf2/ARE pathway has been regarded as a critical switch in the initiation of cellular defence systems for surviving oxidative insults and viral infection. In this study, the Nrf2 gene of EPC cells, which is originally derived from Pimephales promelas, was cloned, and an investigation on the interactions between Nrf2 and spring viraemia of carp virus (SVCV) was performed. These results demonstrated that the virus facilitated the nuclear accumulation of Nrf2 and up-regulated its transcriptional and protein profiles in EPC cells. In addition, exogenous activation of Nrf2 conferred EPC cells with a higher cellular total antioxidant capacity via an increase in the expression of HO-1 and SOD1, but did not suppress the replication of SVCV.

Keywords: EPC; Nrf2; Oxidative stress; SVCV.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Antioxidants / pharmacology
Cyprinidae*
Fish Diseases / genetics*
Fish Diseases / virology
Fish Proteins / chemistry
Fish Proteins / genetics*
Fish Proteins / metabolism
Isothiocyanates / pharmacology
Molecular Sequence Data
NF-E2-Related Factor 2 / chemistry
NF-E2-Related Factor 2 / genetics*
NF-E2-Related Factor 2 / metabolism
Phylogeny
Real-Time Polymerase Chain Reaction / veterinary
Rhabdoviridae / physiology*
Rhabdoviridae Infections / genetics
Rhabdoviridae Infections / veterinary*
Rhabdoviridae Infections / virology
Sequence Alignment / veterinary
Up-Regulation*
Virus Replication / drug effects

Substances

Antioxidants
Fish Proteins
Isothiocyanates
NF-E2-Related Factor 2
sulphoraphene