Optimization of polysaccharides extraction from Clematis huchouensis Tamura and its antioxidant activity

Carbohydr Polym. 2014 Oct 13:111:762-7. doi: 10.1016/j.carbpol.2014.04.103. Epub 2014 May 20.

Abstract

In this study, response surface methodology (RSM), based on Box-Behnken design, was employed to optimize the extraction conditions of polysaccharide from Clematis huchouensis Tamura (CP). And then the antioxidant activities of the samples were investigated including scavenging effects of superoxide and hydroxyl radicals and their reducing power. The results of chemical composition and FT-IR spectrum analysis showed the polysaccharide was acidic proteoglycan. And moreover, CP showed excellent antioxidant activity in these three assays. The purification and structure of CP need to be further studied.

Keywords: Antioxidant activities; Clematis huchouensis Tamura; Polysaccharide; Response surface methodology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / chemistry
  • Antioxidants / isolation & purification*
  • Antioxidants / pharmacology
  • Clematis / chemistry*
  • Hydroxyl Radical / chemistry
  • Plant Extracts / chemistry
  • Plant Extracts / isolation & purification*
  • Plant Extracts / pharmacology
  • Polysaccharides / chemistry
  • Polysaccharides / isolation & purification*
  • Polysaccharides / pharmacology
  • Spectroscopy, Fourier Transform Infrared
  • Superoxides / chemistry

Substances

  • Antioxidants
  • Plant Extracts
  • Polysaccharides
  • Superoxides
  • Hydroxyl Radical