Structural peculiarities of polysaccharide from sterile form of Far Eastern red alga Ahnfeltiopsis flabelliformis

Carbohydr Polym. 2014 Oct 13:111:1-9. doi: 10.1016/j.carbpol.2014.04.022. Epub 2014 Apr 22.

Abstract

KCl-insoluble sulfated polysaccharide from sterile alga Ahnfeltiopsis flabelliformis was investigated. Partial reductive hydrolysis and NMR spectroscopy showed that the polysaccharide comprises disaccharide units of carrabiose only. According to FT-IR-, 1D, 2D NMR spectroscopies and mass-spectrometry this polysaccharide is kappa/beta-carrageenan with ratio of kappa- and beta-types units 3:1 and contains minor amounts of iota- and gamma-carrageenans (precursor of beta-carrageenan). In addition, ESIMS/MS data suggested that xylose (minor amount) is present in the polysaccharide as a substituent one of hydroxyl group of galactose. According to aPTT and PT assays the studied carrageenan affected mostly intrinsic pathway of coagulation, while it effect on the extrinsic pathway is absent.

Keywords: Ahnfeltiopsis flabelliformis; Carrageenan; Mass spectrometry; NMR spectroscopy; Structure.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anticoagulants / chemistry*
  • Anticoagulants / isolation & purification
  • Anticoagulants / pharmacology*
  • Blood Coagulation / drug effects*
  • Carrageenan / chemistry*
  • Carrageenan / isolation & purification
  • Carrageenan / pharmacology*
  • Humans
  • Magnetic Resonance Spectroscopy
  • Mass Spectrometry
  • Partial Thromboplastin Time
  • Prothrombin Time
  • Rhodophyta / chemistry*
  • Spectroscopy, Fourier Transform Infrared

Substances

  • Anticoagulants
  • Carrageenan