Neuronal cell-surface protein neurexin 1 interaction with multi-PDZ domain protein MUPP1

Biosci Biotechnol Biochem. 2014;78(4):644-6. doi: 10.1080/09168451.2014.890031. Epub 2014 Apr 16.

Abstract

Location of membrane proteins is often stabilized by PDZ domain-containing scaffolding proteins. Using the yeast two-hybrid screening, we found that neurexin 1 interacted with multi-PDZ domain protein 1 (MUPP1) through PDZ domain. Neurexin 2 and 3 also interacted with MUPP1. MUPP1 and neurexin 1 were co-localized in cultured cells. These results suggest a novel mechanism for localizing neurexin 1 to synaptic sites.

Keywords: PDZ domain; multi-PDZ domain protein 1 (MUPP1); neurexin; protein–protein interaction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium-Binding Proteins
  • Carrier Proteins / chemistry*
  • Carrier Proteins / metabolism*
  • Membrane Proteins
  • Mice
  • Neural Cell Adhesion Molecules / metabolism*
  • Neurons / cytology*
  • Neurons / metabolism*
  • PDZ Domains*
  • Protein Binding
  • Protein Transport

Substances

  • Calcium-Binding Proteins
  • Carrier Proteins
  • Membrane Proteins
  • Mpdz protein, mouse
  • Neural Cell Adhesion Molecules
  • Nrxn1 protein, mouse