Further validation of unsaturated long-chain fatty acids as inhibitors for oxidized low-density lipoprotein binding to CD36 via assays with synthetic CD36 peptide-cross-linked plates

Biosci Biotechnol Biochem. 2014;78(5):839-42. doi: 10.1080/09168451.2014.891934. Epub 2014 Jun 13.

Abstract

We recently obtained evidence that unsaturated long-chain fatty acids (LCFAs) (e.g. oleic acid) inhibit binding of oxidized low-density lipoproteins (oxLDLs) to CD36. In the present study, we validated this prediction by examining inhibition by unsaturated LCFAs of Alexa-fluor-labeled oxLDL binding to multiwell plates onto which a synthetic CD36 peptide is covalently immobilized via thiol-maleimide coupling.

Keywords: CD36; binding inhibition; oxidized low-density lipoproteins; peptide cross linking; unsaturated long-chain fatty acids.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • CD36 Antigens / chemistry*
  • CD36 Antigens / metabolism*
  • Fatty Acids / chemistry*
  • Fatty Acids / pharmacology*
  • Lipoproteins, LDL / metabolism*
  • Molecular Sequence Data
  • Peptide Fragments / chemistry*
  • Peptide Fragments / metabolism*
  • Protein Binding / drug effects

Substances

  • CD36 Antigens
  • Fatty Acids
  • Lipoproteins, LDL
  • Peptide Fragments
  • oxidized low density lipoprotein