Electrospun nanoporous carbon nanofibers (pCNFs) decorated with Ag-Pt bimetallic nanoparticles have been successfully synthesized by combining template carbonization and seed-growth reduction approach. Porous-structured polyacrylonitrile (PAN) nanofibers (pPAN) were first prepared by electrospinning PAN/polyvinylpyrrolidone (PVP) blend solution, followed by subsequent water extraction and heat treatment to obtain pCNFs. Ag-Pt/pCNFs were then obtained by using pCNFs as support for bimetallic nanoparticle loading. Thus, the obtained Ag-Pt/pCNFs were used to modify glassy carbon electrode (GCE) for selective detection of dopamine (DA) in the presence of uric acid (UA) and ascorbic acid (AA). This novel sensor exhibits fast amperometric response and high sensitivity toward DA with a wide linear concentration range of 10-500 μM and a low detection limit of 0.11 μM (S/N = 3), wherein the interference of UA and AA can be eliminated effectively.