Love-wave sensors combined with microfluidics for fast detection of biological warfare agents

Sensors (Basel). 2014 Jul 15;14(7):12658-69. doi: 10.3390/s140712658.

Abstract

The following paper examines a time-efficient method for detecting biological warfare agents (BWAs). The method is based on a system of a Love-wave immunosensor combined with a microfluidic chip which detects BWA samples in a dynamic mode. In this way a continuous flow-through of the sample is created, promoting the reaction between antigen and antibody and allowing a fast detection of the BWAs. In order to prove this method, static and dynamic modes have been simulated and different concentrations of BWA simulants have been tested with two immunoreactions: phage M13 has been detected using the mouse monoclonal antibody anti-M13 (AM13), and the rabbit immunoglobulin (Rabbit IgG) has been detected using the polyclonal antibody goat anti-rabbit (GAR). Finally, different concentrations of each BWA simulants have been detected with a fast response time and a desirable level of discrimination among them has been achieved.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acoustics / instrumentation*
  • Animals
  • Bacteriophage M13 / isolation & purification*
  • Biological Warfare Agents / classification*
  • Biosensing Techniques / instrumentation*
  • Environmental Monitoring / instrumentation*
  • Equipment Design
  • Equipment Failure Analysis
  • Goats
  • Immunoassay / instrumentation
  • Micro-Electrical-Mechanical Systems / instrumentation*
  • Microfluidic Analytical Techniques / instrumentation*
  • Rabbits
  • Transducers

Substances

  • Biological Warfare Agents