Abstract
A fluorescence method for the detection of DNA methylation and the assay of methyltransferase activity is proposed using gold nanorods as a fluorescence quencher on the basis of fluorescence resonance energy transfer. It is demonstrated that this method is capable of detecting methyltransferase with a detection limit of 0.25 U mL(-1), which might make this method a good candidate for monitoring DNA methylation in the future.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Biosensing Techniques / methods
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DNA Methylation*
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Enzyme Assays / methods*
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Escherichia coli / enzymology*
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Escherichia coli Proteins / analysis
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Escherichia coli Proteins / metabolism*
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Fluorescence Resonance Energy Transfer / methods*
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Gold / chemistry*
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Limit of Detection
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Nanotubes / chemistry*
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Nanotubes / ultrastructure
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Site-Specific DNA-Methyltransferase (Adenine-Specific) / analysis
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Site-Specific DNA-Methyltransferase (Adenine-Specific) / metabolism*
Substances
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Escherichia coli Proteins
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Gold
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Site-Specific DNA-Methyltransferase (Adenine-Specific)
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dam protein, E coli