Molecular, biological, and antigenic characterization of a Border disease virus isolated from a pig during classical swine fever surveillance in Japan

Makoto Nagai; Hiroshi Aoki; Yoshihiro Sakoda; Takashi Kozasa; Kaho Tominaga-Teshima; Junki Mine; Yuri Abe; Tomokazu Tamura; Tsubasa Kobayashi; Kaoru Nishine; Kentaro Tateishi; Yudai Suzuki; Mai Fukuhara; Keitaro Ohmori; Reiko Todaka; Kazuhiko Katayama; Tetsuya Mizutani; Shigeyuki Nakamura; Hiroshi Kida; Junsuke Shirai

doi:10.1177/1040638714541837

Molecular, biological, and antigenic characterization of a Border disease virus isolated from a pig during classical swine fever surveillance in Japan

J Vet Diagn Invest. 2014 Jul;26(4):547-552. doi: 10.1177/1040638714541837.

Authors

Makoto Nagai^{1

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8}, Hiroshi Aoki^{1

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8}, Yoshihiro Sakoda^{1

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8}, Takashi Kozasa^{1

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8}, Kaho Tominaga-Teshima^{1

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8}, Junki Mine^{1

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8}, Yuri Abe^{1

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8}, Tomokazu Tamura^{1

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8}, Tsubasa Kobayashi^{1

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8}, Kaoru Nishine^{1

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8}, Kentaro Tateishi^{1

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8}, Yudai Suzuki^{1

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8}, Mai Fukuhara^{1

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8}, Keitaro Ohmori^{1

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8}, Reiko Todaka^{1

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8}, Kazuhiko Katayama^{1

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8}, Tetsuya Mizutani^{1

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8}, Shigeyuki Nakamura^{1

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8}, Hiroshi Kida^{1

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8}, Junsuke Shirai^{1

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Affiliations

¹ Department of Veterinary Medicine, Faculty of Agriculture (Nagai, Tominaga-Teshima, Tateishi, Suzuki, Fukuhara, Ohmori, Mizutani, Shirai), Tokyo University of Agriculture and Technology, Tokyo, Japan.
² Research and Education Center for Prevention of Global Infectious Diseases of Animals (Nagai, Mizutani, Shirai), Tokyo University of Agriculture and Technology, Tokyo, Japan.
³ School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Tokyo, Japan (Aoki, Kobayashi, Nishine).
⁴ Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan (Sakoda, Mine, Abe, Tamura, Kida).
⁵ Food Safety and Consumer Affairs Bureau (Kozasa).
⁶ National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, Tokyo, Japan (Kozasa, Nakamura).
⁷ Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan (Todaka, Katayama).
⁸ Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan (Kida).

PMID: 25027496
DOI: 10.1177/1040638714541837

Abstract

In the current study, molecular, biological, and antigenic analyses were performed to characterize Border disease virus (BDV) strain FNK2012-1 isolated from a pig in 2012 in Japan. The complete genome comprises 12,327 nucleotides (nt), including a large open reading frame of 11,685 nt. Phylogenetic analysis revealed that FNK2012-1 was clustered into BDV genotype 1 with ovine strains. FNK2012-1 grew in porcine, bovine, and ovine primary cells and cell lines, but grew better in bovine and ovine cells than in porcine cells. Specific pathogen-free pigs inoculated with FNK2012-1 did not show any clinical signs. Noninoculated contact control pigs also did not show clinical signs and did not seroconvert. The results suggest that FNK2012-1 may be of ruminant origin and is poorly adapted to pigs. Such observations can provide important insights into evidence for infection and transmission of BDV, which may be of ruminant origin, among pigs.

Keywords: Border disease virus; pestivirus; swine.

MeSH terms

Animals
Border Disease / virology*
Border disease virus / genetics
Border disease virus / immunology
Border disease virus / physiology*
Genome, Viral*
Phylogeny
RNA, Viral
Sequence Analysis, RNA
Swine
Swine Diseases / virology*

Substances

RNA, Viral