In vitro toxicity evaluation of Ti(4+)-stabilized γ-Bi2O3 sillenites

T Popescu; A R Lupu; M Feder; D Tarabasanu-Mihaila; V S Teodorescu; A M Vlaicu; L Diamandescu

doi:10.1016/j.tiv.2014.07.003

In vitro toxicity evaluation of Ti(4+)-stabilized γ-Bi2O3 sillenites

Toxicol In Vitro. 2014 Dec;28(8):1523-30. doi: 10.1016/j.tiv.2014.07.003. Epub 2014 Jul 12.

Authors

T Popescu¹, A R Lupu², M Feder³, D Tarabasanu-Mihaila³, V S Teodorescu³, A M Vlaicu³, L Diamandescu³

Affiliations

¹ National Institute of Materials Physics, P.O. Box MG-7, Bucharest, Romania; University of Bucharest, Faculty of Physics, Bucharest, Romania. Electronic address: tr.popescu@gmail.com.
² Cantacuzino National Institute of Research and Development for Microbiology and Immunology, Bucharest, Romania.
³ National Institute of Materials Physics, P.O. Box MG-7, Bucharest, Romania.

PMID: 25025181
DOI: 10.1016/j.tiv.2014.07.003

Abstract

We report results regarding the in vitro toxicology of γ-Bi2O3 represented by its isomorphous phase Bi12TiO20 (γ-BTO). The γ-BTO microparticles were synthesized by two methods: coprecipitation from a bismuth nitrate-tetrabutyl titanate solution and solid state reaction of Bi2O3 and TiO2 oxides. The structural and morphological characteristics of the obtained materials were determined using X-ray diffraction (XRD), selected area electron diffraction (SAED), transmission (TEM) and scanning (SEM) electron microscopy. The elemental composition was investigated using energy dispersive spectrometry (EDS). The cytotoxicity and oxidative/nitrosative stress (intracellular reactive oxygen species (ROS) and nitric oxide (NO) release) induced by the studied microparticles in HepG2, SH-SY5Y and 3T3-L1 cell cultures were determined using the MTT, DCF-DA (2',7'-dichlorfluorescein-diacetate) and Griess methods respectively. Depending on the cell type and γ-BTO concentration, results showed only weak cytotoxic effects after 24h of γ-BTO exposure and cell proliferation effects for longer treatment times. Only reduced NO release increases (corresponding to high γ-BTO concentrations) were detected in case of SH-SY5Y and 3T3-L1 cells. The intracellular ROS production (higher for HepG2 cells) appeared inversely proportional to the γ-BTO concentration. The obtained results indicated a promising in vitro biocompatibility of γ-BTO and encourage further studies regarding its potential for biomedical applications.

Keywords: Bi(12)TiO(20); Bismuth oxide; Cytotoxicity; Oxidative stress; Sillenites; γ-Bi(2)O(3).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

3T3-L1 Cells
Animals
Bismuth / toxicity*
Cell Survival / drug effects
Hep G2 Cells
Humans
Mice
Nitric Oxide / metabolism
Reactive Oxygen Species / metabolism
Semiconductors / adverse effects*
Titanium / toxicity*
X-Ray Diffraction

Substances

Reactive Oxygen Species
bismuth titanate
Nitric Oxide
Titanium
Bismuth