Integrin α10β1 is the most abundant collagen-binding integrin in cartilaginous tissues and its expression pattern is distinct from that of other collagen-binding integrins. In vitro and in vivo studies have identified integrin α10β1 as a unique phenotypic marker for chondrocyte differentiation and a crucial mediator of cell-matrix interactions required for proper cartilage development. This chapter describes the structure of the integrin subunit α10, the tissue distribution of the integrin 10β1 and updates available information regarding its regulation and ligand binding. We also summarize current information on the functional roles of α10β1 in chondrogenesis of mesenchymal stem cells and in skeletal growth.