Effect of malic enzyme on ethanol production by Synechocystis sp. PCC 6803

Katsunori Yoshikawa; Takashi Hirasawa; Hiroshi Shimizu

doi:10.1016/j.jbiosc.2014.06.001

Effect of malic enzyme on ethanol production by Synechocystis sp. PCC 6803

J Biosci Bioeng. 2015 Jan;119(1):82-4. doi: 10.1016/j.jbiosc.2014.06.001. Epub 2014 Jul 9.

Authors

Katsunori Yoshikawa¹, Takashi Hirasawa², Hiroshi Shimizu³

Affiliations

¹ Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka 565-0871, Japan; Japan Science and Technology Agency, Core Research for Evolutional Science and Technology (JST, CREST), 1-5 Yamadaoka, Suita, Osaka 565-0871, Japan. Electronic address: yoshikawa@ist.osaka-u.ac.jp.
² Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka 565-0871, Japan; Japan Science and Technology Agency, Core Research for Evolutional Science and Technology (JST, CREST), 1-5 Yamadaoka, Suita, Osaka 565-0871, Japan; Department of Bioengineering, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan. Electronic address: thirasawa@bio.titech.ac.jp.
³ Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka 565-0871, Japan; Japan Science and Technology Agency, Core Research for Evolutional Science and Technology (JST, CREST), 1-5 Yamadaoka, Suita, Osaka 565-0871, Japan. Electronic address: shimizu@ist.osaka-u.ac.jp.

PMID: 25022874
DOI: 10.1016/j.jbiosc.2014.06.001

Abstract

We investigated effects of malic enzyme on ethanol production by Synechocystis sp. PCC 6803 under autotrophic conditions. Deletion of me, which encodes malic enzyme, decreased ethanol production, whereas its overexpression had no effect. Our results suggest that maintaining optimal malic enzyme activity controls ethanol production by Synechocystis sp. PCC 6803.

Keywords: Bioproduction; Cyanobacteria; Ethanol; Malic enzyme; Synechocystis sp. PCC 6803.

MeSH terms

Ethanol / metabolism*
Malate Dehydrogenase / metabolism*
Synechocystis / metabolism*

Substances

Ethanol
Malate Dehydrogenase
malate dehydrogenase (decarboxylating)