Colorimetric estimation of human glucose level using γ-Fe₂O₃ nanoparticles: an easily recoverable effective mimic peroxidase

Biochem Biophys Res Commun. 2014 Aug 15;451(1):30-5. doi: 10.1016/j.bbrc.2014.07.028. Epub 2014 Jul 11.

Abstract

This article reports simple, green and efficient synthesis of γ-Fe₂O₃ nanoparticles (NPs) (maghemite) through single-source precursor approach for colorimetric estimation of human glucose level. The γ-Fe₂O₃ NPs, having cubic morphology with an average particle size of 30 nm, exhibited effective peroxidase-like activity through the catalytic oxidation of peroxidase substrate 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H₂O₂ producing a blue-colored solution. On the basis of this colored-reaction, we have developed a simple, cheap, highly sensitive and selective colorimetric method for estimation of glucose using γ-Fe₂O₃/TMB/glucose-glucose oxidase (GOx) system in the linear range from 1 to 80 μM with detection limit of 0.21 μM. The proposed glucose sensor displays faster response, good stability, reproducibility and anti-interference ability. Based on this simple reaction process, human blood and urine glucose level can be monitored conveniently.

Keywords: Colorimetric glucose determination; Human blood-urine; Hydrogen peroxide; Peroxidase-like activity; γ-Fe(2)O(3) nanoparticles.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Benzidines / chemistry
  • Biomimetics
  • Biosensing Techniques / methods
  • Blood Glucose / analysis
  • Colorimetry / methods*
  • Ferric Compounds / chemical synthesis*
  • Ferric Compounds / chemistry
  • Glucose / analysis*
  • Glycosuria / diagnosis
  • Humans
  • Hydrogen Peroxide / chemistry
  • Male
  • Metal Nanoparticles / chemistry*
  • Oxidation-Reduction
  • Peroxidases / chemistry
  • Reproducibility of Results

Substances

  • Benzidines
  • Blood Glucose
  • Ferric Compounds
  • ferric oxide
  • 3,3',5,5'-tetramethylbenzidine
  • Hydrogen Peroxide
  • Peroxidases
  • Glucose