Induction of CYP1A and ABC transporters in European sea bass (Dicentrarchus labrax) upon 2,3,7,8-TCDD waterborne exposure

Mar Environ Res. 2014 Aug:99:218-22. doi: 10.1016/j.marenvres.2014.06.009. Epub 2014 Jun 30.

Abstract

The aim of this study was to characterize the responsiveness of CYP1A and ABC transport proteins in European Sea bass (Dicentrarchus labrax) waterborne exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) (46 pg/L) for 24 h and 7 days. Genes modulation (abcb1, abcc1-2, cyp1a), EROD activity were investigated in liver and 2,3,7,8-TCDD bioconcentration in liver and muscle. TCDD induced significantly cyp1a gene expression and EROD activity at 24 h and 7 d. A significant up-regulation of abcb1 was also observed but only after 7 days. No modulation of abcc1 and abcc2 genes was observed. Waterborne TCDD exposure was able to induce CYP1A and abcb1 encoding for P-glycoprotein in juvenile of European sea bass.

Keywords: ABC transport proteins; CYP1A; Detoxification; European sea bass; TCDD.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP-Binding Cassette Transporters / metabolism*
  • Analysis of Variance
  • Animals
  • Bass / metabolism*
  • Cytochrome P-450 CYP1A1 / metabolism*
  • DNA Primers / genetics
  • Enzyme Induction / drug effects*
  • Liver / metabolism
  • Muscle, Skeletal / metabolism
  • Polychlorinated Dibenzodioxins / toxicity*
  • Real-Time Polymerase Chain Reaction
  • Time Factors
  • Water Pollutants, Chemical / toxicity*

Substances

  • ATP-Binding Cassette Transporters
  • DNA Primers
  • Polychlorinated Dibenzodioxins
  • Water Pollutants, Chemical
  • Cytochrome P-450 CYP1A1