Ischemic postconditioning relieves cerebral ischemia and reperfusion injury through activating T-LAK cell-originated protein kinase/protein kinase B pathway in rats

Haiping Zhao; Rongliang Wang; Zhen Tao; Li Gao; Feng Yan; Zhi Gao; Xiangrong Liu; Xunming Ji; Yumin Luo

doi:10.1161/STROKEAHA.114.006135

Ischemic postconditioning relieves cerebral ischemia and reperfusion injury through activating T-LAK cell-originated protein kinase/protein kinase B pathway in rats

Stroke. 2014 Aug;45(8):2417-24. doi: 10.1161/STROKEAHA.114.006135. Epub 2014 Jul 10.

Authors

Haiping Zhao¹, Rongliang Wang¹, Zhen Tao¹, Li Gao¹, Feng Yan¹, Zhi Gao¹, Xiangrong Liu¹, Xunming Ji², Yumin Luo²

Affiliations

¹ From the Cerebrovascular Diseases Research Institute (H.Z., R.W., Z.T., F.Y., Z.G., X.L., X.J., Y.L.) and Department of Neurology (L.G.), Xuanwu Hospital of Capital Medical University, Beijing, China; Cerebralvascular Diseases Research Laboratory, Beijing Geriatric Medical Research Center, Beijing, China (H.Z., R.W., Z.T., F.Y., Z.G., X.L., X.J., Y.L.); and Key Laboratory of Neurodegenerative Diseases of Ministry of Education and Beijing Key Laboratory of Translational Medicine for Cerebrovascular Diseases, Beijing, China (H.Z., R.W., Z.T., F.Y., Z.G., X.L., X.J., Y.L.).
² From the Cerebrovascular Diseases Research Institute (H.Z., R.W., Z.T., F.Y., Z.G., X.L., X.J., Y.L.) and Department of Neurology (L.G.), Xuanwu Hospital of Capital Medical University, Beijing, China; Cerebralvascular Diseases Research Laboratory, Beijing Geriatric Medical Research Center, Beijing, China (H.Z., R.W., Z.T., F.Y., Z.G., X.L., X.J., Y.L.); and Key Laboratory of Neurodegenerative Diseases of Ministry of Education and Beijing Key Laboratory of Translational Medicine for Cerebrovascular Diseases, Beijing, China (H.Z., R.W., Z.T., F.Y., Z.G., X.L., X.J., Y.L.). yumin111@ccmu.edu.cn jixm@ccmu.edu.cn.

PMID: 25013016
DOI: 10.1161/STROKEAHA.114.006135

Abstract

Background and purpose: Ischemic postconditioning (IPostC) protects against ischemic brain injury. To date, no study has examined the role of T-LAK-cell-originated protein kinase (TOPK) in IPostC-afforded neuroprotection. We explored the molecular mechanism related with TOPK in antioxidant effect of IPostC against ischemia/reperfusion.

Methods: Focal ischemia was induced in rats by transient middle cerebral artery occlusion. Reactive oxygen species production in the peri-infarct cortex was detected using dihydroethidium. Malondialdehyde, as a marker of lipid peroxidation, and 3-nitrotyrosine, as a marker of protein oxidation, were detected by ELISA. The expression or location of antioxidant proteins and signal molecules TOPK, phosphatase, and tensin homolog, and Akt was analyzed by Western blotting and immunofluorescence.

Results: Our results revealed that IPostC relieved transient middle cerebral artery occlusion-induced oxidative damage by reducing reactive oxygen species, malondialdehyde, and 3-nitrotyrosine accumulation in the peri-infarct cortex and raised levels of antioxidants perioxiredoxin-1, peroxiredoxin-2, and thioredoxin-1. In addition, IPostC increased p-AKT and p-TOPK levels, which colocalized in neural cells. In vitro TOPK knockdown by small interfering RNA decreased the levels of antioxidants peroxiredoxin-1, thioredoxin, and manganese superoxide dismutase activity in PC12 cells. In vivo intracerebroventricular injection of TOPK small interfering RNA reversed IPostC-induced neuroprotection by increasing infarct volume and nitric oxide content and reducing manganese superoxide dismutase activity. Moreover, IPostC-evoked Akt activation was blocked by TOPK small interfering RNA in vivo, but the decreased phosphorylated phosphatase and tensin homolog level in ischemia/reperfusion was not influenced by IPostC or by TOPK small interfering RNA treatment.

Conclusions: Our results suggest that the antioxidative effects of TOPK/Akt might contribute to the neuroprotection of IPostC treatment against transient middle cerebral artery occlusion.

Keywords: T-LAK cell-originated protein kinase; brain ischemia; ischemic postconditioning; oxidative stress.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brain / metabolism*
Brain Ischemia / metabolism*
Ischemic Postconditioning*
Lipid Peroxidation / physiology
Malondialdehyde / metabolism
Mitogen-Activated Protein Kinase Kinases / metabolism*
Phosphorylation
Proto-Oncogene Proteins c-akt / metabolism*
Rats
Reactive Oxygen Species / metabolism
Reperfusion Injury / metabolism
Reperfusion Injury / prevention & control*
Signal Transduction / physiology
Superoxides / metabolism

Substances

Reactive Oxygen Species
Superoxides
Malondialdehyde
Proto-Oncogene Proteins c-akt
Mitogen-Activated Protein Kinase Kinases
PDZ-binding kinase