A fluorescent probe for site I binding and sensitive discrimination of HSA from BSA

Chem Commun (Camb). 2014 Aug 28;50(67):9573-6. doi: 10.1039/c4cc03778b.

Abstract

A fluorescent probe DH1 has been successfully developed to detect HSA via site I non-covalent bonding. DH1 shows a dramatic fluorescence enhancement towards HSA without interference from other proteins. The molecular docking method, for the first time, was utilized to provide deep insight into the sensing mechanism of the probe. Moreover, probe DH1 was successfully used to detect trace HSA in healthy human urine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Benzopyrans / chemistry*
  • Binding Sites
  • Cattle
  • Chemistry Techniques, Analytical / methods*
  • Fluorescent Dyes / chemistry*
  • Humans
  • Molecular Docking Simulation
  • Protein Conformation
  • Serum Albumin, Bovine / analysis*
  • Serum Albumin, Bovine / chemistry*

Substances

  • Benzopyrans
  • Fluorescent Dyes
  • dicyanomethylene-4H-chromene
  • Serum Albumin, Bovine