The first naturally occurring trimeric anthracene derivatives, stemphylanthranols A and B (1 and 2), were obtained from the endophytic fungus Stemphylium globuliferum that had been isolated from Juncus actus growing in Egypt. The structures of the new compounds were unambiguously determined by 1D and 2D NMR, and by HRMS. A hypothetical biosynthetic pathway for the new trimers is proposed.